Diverse set of microRNAs are responsive to powdery mildew infection and heat stress in wheat (Triticum aestivum L.)
Diverse set of microRNAs are responsive to powdery mildew infection and heat stress in wheat (Triticum aestivum L.)
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多种 microRNA 对小麦 (Triticum aestivum L.) 的白粉病感染和热应激有反应
DOI：
10.1186/1471-2229-10-123
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发表时间：
2010-06-24
影响因子：
5.3
通讯作者：
Sun, Qixin
中科院分区：
文献类型：
--
作者：
Xin, Mingming;Wang, Yu;Sun, Qixin
背景资料：microRNA（miRNAs）是一类非编码小分子调控RNA，通过引导靶mRNA切割或翻译抑制来调控基因表达。结果：为了验证miRNAs是否参与调节小麦对白粉病和热胁迫的反应，利用Solexa高通量测序技术，从小麦优势生理菌株Erysiphe graminis f. sp. tripenum（Egt）或通过热应激处理。共鉴定出153个miRNA，分别属于51个已知的miRNA家族和81个新的miRNA家族。我们发现24和12个miRNAs分别对白粉病感染和热胁迫有反应。我们进一步预测了149个靶基因可能受到新的小麦miRNAs的调控。结论：我们的研究结果表明，不同的小麦miRNAs对白粉病感染和热胁迫都有反应，并且可能在小麦对生物和非生物胁迫的反应中发挥作用。
Background: MicroRNAs (miRNAs) are a class of small non-coding regulatory RNAs that regulate gene expression by guiding target mRNA cleavage or translational inhibition. MiRNAs can have large-scale regulatory effects on development and stress response in plants.Results: To test whether miRNAs play roles in regulating response to powdery mildew infection and heat stress in wheat, by using Solexa high-throughput sequencing we cloned the small RNA from wheat leaves infected by preponderant physiological strain Erysiphe graminis f. sp. tritici (Egt) or by heat stress treatment. A total of 153 miRNAs were identified, which belong to 51 known and 81 novel miRNA families. We found that 24 and 12 miRNAs were responsive to powdery mildew infection and heat stress, respectively. We further predicted that 149 target genes were potentially regulated by the novel wheat miRNA.Conclusions: Our results indicated that diverse set of wheat miRNAs were responsive to powdery mildew infection and heat stress and could function in wheat responses to both biotic and abiotic stresses.