The expression of matrix metalloproteinase 9 and cathepsin B in gastric carcinoma is associated with lymph node metastasis, but not with postoperative survival
The expression of matrix metalloproteinase 9 and cathepsin B in gastric carcinoma is associated with lymph node metastasis, but not with postoperative survival
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DOI：
10.2478/v10042-008-0007-6
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发表时间：
2008-01-01
影响因子：
1.5
通讯作者：
Bandurski, Roman
中科院分区：
文献类型：
--
作者：
Czyzewska, Jolanta;Guzinska-Ustymowicz, Katarzyna;Bandurski, Roman
基底膜的降解成分可能是肿瘤侵袭的关键。半胱氨酸蛋白酶在这一过程中起着关键作用，即组织蛋白和基质金属蛋白酶。本研究的目的是探讨胃癌组织中基质金属蛋白酶-9和组织蛋白酶B的表达与肿瘤侵袭性、淋巴结转移和生存率的关系。制备91例胃癌患者5MU厚的连续切片，用抗人单抗(NCL-MMP9克隆；稀释度1：40和NCL-Cath-B克隆；稀释度1：40)检测MMP9和Cathepsin B的表达。对患者进行84个月的临床监测。MMP9和组织蛋白酶B在肿瘤主要肿块中的表达与患者性别、肿瘤部位、Lauren‘s分级和组织分化程度无关。肿瘤主要肿块中基质金属蛋白酶-9的表达与肿瘤侵袭深度无关。MMP9和组织蛋白酶B的表达与肿瘤主要肿块和淋巴结转移密切相关(p<；0.001；p<；0.001)。然而，我们没有观察到肿瘤主要肿块中组织蛋白酶B和基质金属蛋白酶9的表达与淋巴结转移和5年总生存期之间的关系。提示基质金属蛋白酶-9(MMP9)和组织蛋白酶B的表达与进展期胃癌的淋巴结转移有关，而与患者的术后生存无关。
Degradation components of basement membrane could be crucial for tumor invasion. A key role in this process has been assigned to cysteine proteases, i.e. cathepsins and matrix metalloproteinases. The purpose of this study was to investigate the relationship of the expression of MMP-9 and cathepsin B with tumor aggressiveness expressed by lymph node metastases and survival rates in gastric carcinoma patients. Slides of 5 mu m-thick serial sections from 91 patients with primary gastric carcinoma were prepared and analyzed for MMP-9 and cathepsin B expression using anti-human monoclonal antibody (NCL-MMP-9 clone; dilution 1:40 and NCL-CATH-B clone; dilution 1:40). The patients were clinically monitored for 84 months. We found no association between the expression of MMP-9 and cathepsin B in main mass of tumor and patients' gender, tumor location, Lauren's classification or histological differentiation. Also no correlation was observed between the expression of MMP-9 in main mass of tumor and depth of invasion. A strong statistically significant association was found between the expression of MMP-9 and cathepsin B in main mass of tumor and lymph node involvement (p< 0.001; p< 0.001, respectively). However, we observed no correlation between the expression of MMP-9 and cathepsin B in main mass of tumor and lymph node involvement or 5-year overall survival. Our results may suggest that the expression of matrix metalloproteinase-9 (MMP-9) and cathepsin B is correlated with lymph node metastasis in advanced gastric carcinoma, but not with patients' postoperative survival.