Bacterial MgrB peptide activates chemoreceptor Fpr3 in mouse accessory olfactory system and drives avoidance behaviour
Bacterial MgrB peptide activates chemoreceptor Fpr3 in mouse accessory olfactory system and drives avoidance behaviour
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DOI：
10.1038/s41467-019-12842-x
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发表时间：
2019-10-25
影响因子：
16.6
通讯作者：
Zufall, Frank
中科院分区：
文献类型：
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作者：
Bufe, Bernd;Teuchert, Yannick;Zufall, Frank
甲酰基肽受体（Fpr）家族的天然免疫化学受体由副嗅觉系统中的犁鼻感觉神经元（VSNs）表达。它们的生物学功能和编码机制仍然未知。我们发现，小鼠Fpr 3（Fpr-rs 1）识别的核心肽基序f-MKKFRW，主要存在于信号序列的细菌蛋白质MgrB，一个高度保守的调节器的毒力和耐药性的肠杆菌科。MgrB肽可以由细菌产生和分泌，并且被VSN的子集选择性地识别。暴露于肽也刺激VSN在自由行为的小鼠和驱动先天回避。我们的数据表明，Fpr 3是必需的神经元检测和避免肽来自一个保守的主毒力调节肠道细菌。
Innate immune chemoreceptors of the formyl peptide receptor (Fpr) family are expressed by vomeronasal sensory neurons (VSNs) in the accessory olfactory system. Their biological function and coding mechanisms remain unknown. We show that mouse Fpr3 (Fpr-rs1) recognizes the core peptide motif f-MKKFRW that is predominantly present in the signal sequence of the bacterial protein MgrB, a highly conserved regulator of virulence and antibiotic resistance in Enterobacteriaceae. MgrB peptide can be produced and secreted by bacteria, and is selectively recognized by a subset of VSNs. Exposure to the peptide also stimulates VSNs in freely behaving mice and drives innate avoidance. Our data shows that Fpr3 is required for neuronal detection and avoidance of peptides derived from a conserved master virulence regulator of enteric bacteria.