Components of the Arabidopsis C-repeat/dehydration-responsive element binding factor cold-response pathway are conserved in Brassica napus and other plant species
Components of the Arabidopsis C-repeat/dehydration-responsive element binding factor cold-response pathway are conserved in Brassica napus and other plant species
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DOI：
10.1104/pp.010548
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发表时间：
2001-11-01
期刊：
影响因子：
7.4
通讯作者：
Thomashow, MF
中科院分区：
文献类型：
--
作者：
Jaglo, KR;Kleff, S;Thomashow, MF
许多植物提高了对低温的耐寒能力，这种现象被称为冷驯化。拟南芥的冷驯化包括在冷诱导下快速表达C-重复/脱水反应元件结合因子(CBF)转录激活子，然后表达CBF靶向基因以提高耐寒性。在这里，我们提出了在甘蓝型油菜中存在CBF冷反应途径的证据。我们发现甘蓝型油菜编码CBF类基因，这些基因的转录产物对低温的响应迅速积累，紧随其后的是冷调节Bn115基因的表达，Bn115基因是以CBF为靶标的拟南芥COR15a基因的同源基因。此外，我们还发现，在转CBF基因的甘蓝型油菜植株中结构性过表达CBF基因可以诱导拟南芥CBF靶向基因的同源基因的表达，并提高未驯化和冷驯化植株的耐寒性。低温诱导的小麦(Triticum aestivum L.cv Norstar)和黑麦(Secale graale L.cv Puma)以及番茄(Lycopersicon Esculentum Var)中编码类CBF蛋白的转录本也迅速积累。Bonny Best、Castle Mart、Micro-Tom和D Huang)，一种不冷适应的冰冻敏感植物。对拟南芥、甘蓝型油菜、小麦、黑麦和番茄CBF蛋白的比对表明，它们存在保守的氨基酸序列PKK/RPAGRxKFxETRHP和DSAWR，它们包围了这些蛋白的AP2/EREBP DNA结合域，并将它们与AP2/EREBP蛋白家族的其他成员区分开来。我们的结论是，CBF冷反应途径的组成部分在开花植物中高度保守，并不局限于那些冷适应的植物。
Many plants increase in freezing tolerance in response to low, nonfreezing temperatures, a phenomenon known as cold acclimation. Cold acclimation in Arabidopsis involves rapid cold-induced expression of the C-repeat/dehydration-responsive element binding factor (CBF) transcriptional activators followed by expression of CBF-targeted genes that increase freezing tolerance. Here, we present evidence for a CBF cold-response pathway in Brassica napus. We show that B. napus encodes CBF-like genes and that transcripts for these genes accumulate rapidly in response to low temperature followed closely by expression of the cold-regulated Bn115 gene, an ortholog of the Arabidopsis CBF-targeted COR15a gene. Moreover, we show that constitutive overexpression of the Arabidopsis CBF genes in transgenic B. napus plants induces expression of orthologs of Arabidopsis CBF-targeted genes and increases the freezing tolerance of both nonacclimated and cold-acclimated plants. Transcripts encoding CBF-like proteins were also found to accumulate rapidly in response to low temperature in wheat (Triticum aestivum L. cv Norstar) and rye (Secale cereale L. cv Puma), which cold acclimate, as well as in tomato (Lycopersicon esculentum var. Bonny Best, Castle Mart, Micro-Tom, and D Huang), a freezing-sensitive plant that does not cold acclimate. An alignment of the CBF proteins from Arabidopsis, B. napus, wheat, rye, and tomato revealed the presence of conserved amino acid sequences, PKK/RPAGRxKFxETRHP and DSAWR, that bracket the AP2/EREBP DNA binding domains of the proteins and distinguish them from other members of the AP2/EREBP protein family. We conclude that components of the CBF cold-response pathway are highly conserved in flowering plants and not limited to those that cold acclimate.