Aedes aegypti salivary protein "aegyptin" co-inoculation modulates dengue virus infection in the vertebrate host
Aedes aegypti salivary protein "aegyptin" co-inoculation modulates dengue virus infection in the vertebrate host
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DOI：
10.1016/j.virol.2014.07.019
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发表时间：
2014-11-01
期刊：
影响因子：
3.7
通讯作者：
Mores, C. N.
中科院分区：
文献类型：
--
作者：
McCracken, M. K.;Christofferson, R. C.;Mores, C. N.
登革病毒（DENV）在埃及伊蚊吸血时通过其唾液传播。这种唾液由许多蛋白质组成，这些蛋白质能够破坏止血过程或调节脊椎动物的免疫反应。一种名为“埃及伊蚊蛋白”的蛋白质是一种过敏原和凝血形成抑制剂，并且在感染登革病毒的蚊子唾液中含量降低。为了研究埃及伊蚊蛋白对脊椎动物登革病毒感染的影响，我们给IRF - 3/7（-/- -/-）小鼠接种登革病毒2型1232株，同时或不同时接种埃及伊蚊蛋白。与仅接种登革病毒的小鼠相比，接种埃及伊蚊蛋白的小鼠在接种部位和血液中的登革病毒滴度降低，并且在接种后48小时粒细胞 - 巨噬细胞集落刺激因子（GM - CSF）、干扰素 - γ（IFN - γ）、白细胞介素 - 5（IL - 5）和白细胞介素 - 6（IL - 6）的浓度升高。这些以及其他数据表明，埃及伊蚊蛋白通过增强免疫反应的诱导来影响登革病毒的持续存在。（C）2014爱思唯尔公司。保留所有权利。
Dengue virus (DENV) is transmitted in the saliva of the mosquito vector Aedes aegypti during blood meal acquisition. This saliva is composed of numerous proteins with the capacity to disrupt hemostasis or modulate the vertebrate immune response. One such protein, termed "aegyptin," is an allergen and inhibitor of clot formation, and has been found in decreased abundance in the saliva of DENV-infected mosquitoes. To examine the influence of aegyptin on DENV infection of the vertebrate, we inoculated IRF-3/7(-/- -/-) mice with DENV serotype 2 strain 1232 with and without co-inoculation of aegyptin. Mice that received aegyptin exhibited decreased DENV titers in inoculation sites and in circulation, as well as increased concentrations of GM-CSF, IFN-gamma, IL-5, and IL-6, at 48 h post-inoculation when compared to mice that received inoculation of DENV alone. These and other data suggest that aegyptin impacts DENV perpetuation via elevated induction of the immune response. (C) 2014 Elsevier Inc. All rights reserved.