AUTOCRINE REGULATION OF RHEUMATOID ARTHRITIS SYNOVIAL CELL GROWTH IN-VITRO
AUTOCRINE REGULATION OF RHEUMATOID ARTHRITIS SYNOVIAL CELL GROWTH IN-VITRO
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DOI：
10.1016/1043-4666(90)90009-i
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发表时间：
1990-01-01
期刊：
影响因子：
3.8
通讯作者：
MOORE M E
中科院分区：
文献类型：
--
作者：
GODDARD D H;GROSSMAN S L;MOORE M E
类风湿性关节炎(RA)而不是骨关节炎(OA)滑膜细胞在无血清培养液中增殖，这一发现表明，在体外，RA滑膜细胞可能通过持续自分泌至少一种多肽生长因子而被刺激生长。加入单抗1D11.16或兔多克隆抗肿瘤生长因子β。(抗转化生长因子-β。)在培养中，针对RA滑膜细胞的抗体(包括抗转化生长因子β1和转化生长因子β2的中和抗体)导致细胞生长显著减少，当在培养上清液中加入其他生长因子抗体(血小板衍生生长因子[PDGF]、表皮生长因子[EGF]或EGF受体)时，这种影响是看不到的。综上所述，这些数据与RA滑膜细胞体外生长是由内源性转化生长因子-β驱动的观点是一致的。此外，当在培养液中添加EGF时，RA而不是OA的滑膜细胞数量显著增加。这一发现表明，RA滑膜细胞处于细胞周期的G1期；这一效应可能是由内源性转化生长因子-β介导的。
Rheumatoid arthrities (RA), and not osteoarthritis (OA) synovial cells proliferate in serum-free medium, a finding that suggest that, in vitro, RA synovial cells may be stimulated to grow by the continuous autocrine production of at least one polypeptide growth factor. Adding monoclonal antibody 1D11.16, or rabbit polyclonal anti-tumor growth factor .beta. (anti-TGF-.beta.) antibodies (both neutralizing antibodies to TGF-.beta.1 and TGF-.beta.2) to RA synovial cells, in culture, caused a significant reduction in cell growth, an effect not seen when other growth factor antibodies (platelet-derived growth factor [PDGF], epidermal growth factor [EGF], or EGF receptor) were added to the culture medium. Taken together, these data are consistent with the concept that RA synovial cell growth in vitro is driven endogenous TGF-.beta... Moreover, when EGF was added to the culture medium, this caused the numbers of RA, and not OA, synovial cells to increase significantly. This finding suggest that RA synovial cells are in G1 phase of the cell cycle; an effect that could be mediated by endogenous TGF-.beta.