Denaturing gradient gel electrophoresis (DGGE)-based monitoring of intestinal lactobacilli and bifidobacteria of pigs during a feeding trial
Denaturing gradient gel electrophoresis (DGGE)-based monitoring of intestinal lactobacilli and bifidobacteria of pigs during a feeding trial
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DOI：
10.1080/17450390902733959
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发表时间：
2009-01-01
影响因子：
2
通讯作者：
Kneifel, Wolfgang
中科院分区：
文献类型：
--
作者：
Petersson, Agnes;Domig, Konrad J.;Kneifel, Wolfgang
本研究旨在确定不同饲养策略对有机生长肥育猪肠道菌群的影响。将76头猪分配到4个不同的日粮处理组（对照组、益生菌组、玉米青贮组和草青贮组）。监测所施用的益生菌制剂对肠道和粪便微生物群组成的影响。通过使用基于DGGE（变性梯度凝胶电泳）的方法，获得了肠道微生物群的指纹图谱。从粪便和结肠样品中提取总微生物DNA，并使用不同的引物组进行PCR扩增以检测双歧杆菌和乳酸杆菌。使用DGGE分离PCR产物，并将所得图谱与其他饮食处理的结果进行比较。从凝胶上切下条带并测序以进一步鉴定。特别是两个不同的DGGE谱的双歧杆菌观察到，而乳酸杆菌显示出更大的品种内的饮食治疗。
The aim of this study was to determine the influence of different feeding strategies on the gut microbiota of organic growing-finishing pigs. A total of 76 pigs were allocated to four different dietary treatments (control, probiotics, maize silage and grass silage). Effects of the applied probiotic preparation on the composition of the intestinal and faecal microbiota were monitored. By using a DGGE (denaturing gradient gel electrophoresis)-based methodology, fingerprints of the intestinal microbiota were obtained. The total microbial DNA was isolated from faecal and colon samples and amplified with PCR using different primer sets to detect bifidobacteria and lactobacilli. PCR products were separated using DGGE and the resulting profiles were compared with the findings of the other dietary treatments. Bands were excised from the gels and sequenced for further identification. Particularly two different DGGE profiles of bifidobacteria were observed, while lactobacilli showed larger variety within the dietary treatments.