Epigenetic Alteration of the Wnt Inhibitory Factor-1 Promoter Is Common and Occurs in Advanced Stage of Tunisian Nasopharyngeal Carcinoma
Epigenetic Alteration of the Wnt Inhibitory Factor-1 Promoter Is Common and Occurs in Advanced Stage of Tunisian Nasopharyngeal Carcinoma
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DOI：
10.3109/07357907.2010.494324
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发表时间：
2010-11-01
影响因子：
2.4
通讯作者：
Mokdad-Gargouri, Raja
中科院分区：
文献类型：
--
作者：
Fendri, Ali;Khabir, Abdelmajid;Mokdad-Gargouri, Raja
无翼型 (Wnt) 信号通路的激活在癌症中很常见。 Wnt 抑制因子-1 (WIF-1) 是一种分泌型拮抗剂，通过与 Wnt 配体结合发挥作用。我们通过甲基化特异性 PCR (MSP) 检查了 68 例鼻咽癌 (NPC) 和 10 例正常粘膜中 WIF-1 是否失活。我们发现，89.7% 的肿瘤中 WIF-1 启动子被甲基化，而所有正常粘膜均未甲基化。 WIF-1 甲基化与肿瘤、淋巴结和转移 (TNM) (p = .003) 以及年龄 (p = .014) 相关。 Wnt-5a mRNA 在肿瘤中较高，并且与 TNM 相关 (p = .012)。 WIF-1 的甲基化有助于激活 NPC 中的 Wnt 通路。
Activation of the wingless-type (Wnt) signaling pathway is common in cancers. The Wnt inhibitory factor-1 (WIF-1) is a secreted antagonist that acts by binding to Wnt ligands. We examined by methylation-specific PCR (MSP), whether WIF-1 is inactivated in 68 nasopharyngeal carcinomas (NPC), and 10 normal mucosa. We showed that the WIF-1 promoter was methylated in 89.7% of tumors, whereas all normal mucosa were unmethylated. The WIF-1 methylation was associated with the tumor, node, and metastasis (TNM) (p = .003) and the age (p = .014). The Wnt-5a mRNA was higher in tumors and correlated with TNM (p = .012). The methylation of WIF-1 contributes to the activation of the Wnt pathway in NPC.