EMT is not required for lung metastasis but contributes to chemoresistance-猫眼课题宝

DOI：

发表时间：

2016

期刊：

The Science of the total environment

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通讯作者：

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其他

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作者：

Kari R. Fischer;A. Durrans;Sharrell B Lee;J. Sheng;Fuhai Li;Stephen T. C. Wong;Hyejin Choi;Tina El Raye

上皮间质转化（EMT）在转移中的作用是一个长期存在争议的来源，主要是由于无法在体内监测瞬时和可逆的EMT表型。我们建立了一个EMT谱系追踪系统来监测这一过程，在自发性乳腺癌中使用间充质特异性Cre介导的荧光标记开关系统。出于学术研究的目的，用户可以查看、打印、复制和下载此类文件中的文本和数据挖掘内容，但始终要遵守完整的用途：http://www.nature.com/authors/editorial_policies/license.html#terms通信地址：Dingcheng Gao，dig2009@med.cornell.edu，Vivek Mittal，vim2010@med.cornell.edu。作者投稿：D.G.，K.R.F.，和VM。设计实验。KRF还有D.G.进行了实验。公元，S.L.公司，华盛顿特区，T. E. R和S. R提供实验和动物工作的技术支持。L.T.V.和N.K.A.提出批评意见，以改善研究设计。J.S.，F. L.，和S.W.进行RNA测序分析。J.T.，和RFS产生Vim-Cre转基因小鼠。K.R.F.，丹吉和VM。在其他作者的帮助下撰写并编辑了这份手稿。所有作者讨论了结果和从中得出的结论。经济利益冲突：作者声明没有经济利益冲突。HHS公共访问作者手稿自然。作者手稿;可在PMC 2016年5月26日。以最终编辑形式发表：自然。2015年11月26日; 527（7579）：472-476。doi：10.1038/nature15748. A uhor M anscript

The role of epithelial to mesenchymal transition (EMT) in metastasis is a longstanding source of controversy, largely due to an inability to monitor transient and reversible EMT phenotypes in vivo. We established an EMT lineage tracing system to monitor this process, using a mesenchymal-specific Cre-mediated fluorescent marker switch system in spontaneous breast-toUsers may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms Correspondence to: Dingcheng Gao, dig2009@med.cornell.edu, Vivek Mittal, vim2010@med.cornell.edu. Author Contributions: D.G., K.R.F., and V.M. designed the experiments. K.R.F. and D.G. performed the experiments. A.D., S.L., H.C., T. E. R and S.R., provided technical support with experiments and animal work. L.T.V. and N.K.A. made critical comments to improve the study design. J.S., F.L., and S.W. performed RNA-sequencing analysis. J.T., and R.F.S. generated the Vim-Cre transgenic mice. K.R.F., D.G. and V.M. wrote and edited the manuscript with input from the other authors. All authors discussed the results and conclusions drawn from them. Competing financial interests: The authors declare no competing financial interests. HHS Public Access Author manuscript Nature. Author manuscript; available in PMC 2016 May 26. Published in final edited form as: Nature. 2015 November 26; 527(7579): 472–476. doi:10.1038/nature15748. A uhor M anscript

EMT is not required for lung metastasis but contributes to chemoresistance

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