The SMALL AND ROUND SEED1 (SRS1/DEP2) gene is involved in the regulation of seed size in rice
The SMALL AND ROUND SEED1 (SRS1/DEP2) gene is involved in the regulation of seed size in rice
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DOI：
10.1266/ggs.85.327
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发表时间：
2010-10-01
影响因子：
1.1
通讯作者：
Iwasaki, Yukimoto
中科院分区：
文献类型：
--
作者：
Abe, Yuki;Mieda, Keiko;Iwasaki, Yukimoto
利用图位克隆技术从水稻中克隆到一个新的小而圆的种子突变体1（srs 1）的致病基因，命名为小而圆的种子1（SRS 1）。SRS 1基因与先前鉴定的密集和直立PANICLE 2（DEP 2）相同。SRS 1/DEP 2基因编码一个1365个氨基酸残基的新蛋白，没有已知的功能结构域。在外稃的纵向上，与野生型相比，srs 1 -1的细胞长度和细胞数量都减少了，而在外稃的横向横截面上，srs 1 -1的细胞长度大于野生型，但srs 1 -1的细胞数量与野生型相同。这些结果表明，srs 1 -1的小而圆的种子表型是由于细胞长度和细胞数量在纵向方向上的减少，以及细胞在横向方向上的伸长。SRS 1基因在野生型水稻幼叶、节间和幼穗中表达丰富。有趣的是，表达SRS 1的组织与SRS 1突变体中表现出异常的组织密切相关。
The causal gene of a novel small and round seed mutant 1 (srs1) was identified in rice by map-based cloning and named SMALL AND ROUND SEED 1 (SRS1). The SRS1 gene is identical to the previously identified DENSE AND ERECT PANICLE 2 (DEP2). The SRS1/DEP2 gene encodes a novel protein of 1365 amino acids residues without known functional domains. In the longitudinal direction of the lemma, both cell length and cell number are reduced in srs1-1 compared to the wild type, whereas in the lateral cross section of the lemma, cell length in srs1-1 is greater than that in the wild type, but the cell number in srs1-1 is the same as that in wild type. These results suggest that the small and round seed phenotype of srs1-1 is due to the reduction in both cell length and cell number in the longitudinal direction, and the elongation of the cells in the lateral direction of the lemma. The SRS1 mRNA and proteins are abundant in wild type rice specifically in young organs, namely young leaves, internodes and panicles. Interestingly, the tissues expressing SRS1 are closely related to the tissues that exhibit abnormalities in the srs1 mutants.