Gene cloning and expression patterns of two prophenoloxidases from Catantops pinguis (Orthoptera: Catantopidae).
Gene cloning and expression patterns of two prophenoloxidases from Catantops pinguis (Orthoptera: Catantopidae).
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DOI：
10.1017/s0007485312000831
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发表时间：
2013-08
影响因子：
1.9
通讯作者：
Huizhen Zheng;Lingshun Li;Qi Xu;Q. Zou;B. Tang;Shigui Wang
中科院分区：
文献类型：
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作者：
Huizhen Zheng;Lingshun Li;Qi Xu;Q. Zou;B. Tang;Shigui Wang
昆虫脂肪体在昆虫天然免疫中起着重要作用。酚氧化酶（Phenoloxidase，PO）是昆虫天然免疫的重要组成部分，是适应环境所必需的。本研究从斑腿马唐Catantops pinguis（Stål）的脂肪体中分离得到两个多酚氧化酶原（prophenoloxidase，PPO）亚基。其中一个亚基（CpPPO 1）全长2347 bp，开放阅读框（ORF）2187 bp，编码728个氨基酸;另一个亚基（CpPPO 2）全长2445 bp，编码691个氨基酸。这两个PPO基因产物被预测具有所有的结构特征的其他PPO成员，包括两个推定的酪氨酸酶铜结合基序与6个高度保守的组氨酸残基和硫酯样基序。组织分布分析表明，这两种PPO mRNA在11个组织中的脂肪体中大量表达，并且在大肠杆菌感染后瞬时上调，与它们是免疫应答基因一致。CpPPO 1和CpPPO 2 mRNA转录的总水平在一龄幼虫和成虫中要高得多。CpPPO 1的转录水平在几个月内检测到更高，而CpPPO 2的mRNA表达水平在1月，7月，10月和12月极高。上述结果表明，脂肪体中的多酚氧化酶可能在青蛤的生长发育和驯化过程中发挥重要作用。pinguis。
In insect, fat body plays major roles in insect innate immunity. Phenoloxidase (PO) is an important component in insect innate immunity and is necessary for acclimatization. In our study, two prophenoloxidase (PPO) subunits were obtained from fat body of Catantops pinguis (Stål). The full-length cDNA sequence of one PPO (CpPPO1) consisted of 2347 bp with an open reading frame (ORF) of 2187 bp encoding 728 amino acids, while the other subunit (CpPPO2) had a full length of 2445 bp, encoding 691 amino acids. Both the PPO gene products are predicted to possess all the structural features of other PPO members, including two putative tyrosinase copper-binding motifs with six highly conserved histidine residues and a thiolester-like motif. Tissue distribution analysis showed that both PPO mRNAs were abundantly expressed in the fat body among 11 tissues examined, and they were transiently up-regulated after Escherichia coli infection, consistent with them being immune-responsive genes. Total levels of CpPPO1 and CpPPO2 mRNA transcripts were much higher in first instar larvae and adults. A much higher transcript level of CpPPO1 was detected in several months, while there were extremely high mRNA expression levels of CpPPO2 in January, July, October, and December. The above results suggested that PPO from fat body might also bring significant function during the processes of development and acclimatization for C. pinguis.