ANTIMICROBIAL RESISTANCE PATTERNS AND PLASMID PROFILES OF STREPTOCOCCUS-SUIS ISOLATES
ANTIMICROBIAL RESISTANCE PATTERNS AND PLASMID PROFILES OF STREPTOCOCCUS-SUIS ISOLATES
复制标题
DOI：
10.1177/104063879200400209
复制
发表时间：
1992-04-01
影响因子：
1.5
通讯作者：
GOTTSCHALK, M
中科院分区：
文献类型：
--
作者：
CANTIN, M;HAREL, J;GOTTSCHALK, M
从魁北克和加拿大西部的患病动物以及欧洲的人类病例中回收的猪链球菌分离株对不同抗菌剂的敏感性进行了测试，并筛选了它们的质粒含量。来自魁北克的大多数分离株对克林霉素、红霉素和四环素具有耐药性;来自加拿大西部的动物分离株对克林霉素和红霉素的耐药性明显较低，而人类分离株对大多数测试的抗生素的敏感性则高得多。在发现的7种质粒谱中，2种在来自魁北克和加拿大西部的分离株中特别常见，表明猪群中存在流行菌株。一个特定的质粒带约5 kb的存在于大多数加拿大分离株。当该带被用作菌落和Southern印迹杂交中的探针时，大多数携带5-kb质粒的分离物杂交，即使它们的质粒谱不同。来自欧洲的人类分离株的质粒含量与来自加拿大的动物来源的分离株不同。虽然在大多数分离株中，高度的抗菌素耐药性与质粒的存在有关，但不可能建立因果关系。
Streptococcus suis isolates recovered from diseased animals in Quebec and western Canada and from human cases in Europe were tested for their susceptibility to different antimicrobial agents and screened for their plasmid content. Most isolates from Quebec were clindamycin, erythromycin, and tetracycline resistant; animal isolates from western Canada were notably less resistant to clindamycin and erythromycin, whereas human isolates were considerably more susceptible to most antimicrobials tested.More than 60% of isolates had plasmids that ranged from 1.5 to 35 kilobases (kb). Of the 7 plasmid profiles found, 2 were particularly frequent in isolates from Quebec and western Canada, suggesting the presence of epidemic strains in the swine population. A particular plasmid band of about 5 kb was present in most Canadian isolates. When this band was used as a probe in colony and Southern blot hybridization, most isolates harboring the 5-kb plasmid hybridized, even though their plasmid profiles were different. Human isolates from Europe differed in their plasmid content from Canadian isolates of animal origin. Although a high degree of antimicrobial resistance was associated with the presence of plasmids in most isolates, it was not possible to establish a causative relationship.