Characterization of Small RNAs Derived from tRNAs, rRNAs and snoRNAs and Their Response to Heat Stress in Wheat Seedlings.
Characterization of Small RNAs Derived from tRNAs, rRNAs and snoRNAs and Their Response to Heat Stress in Wheat Seedlings.
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小麦幼苗中 tRNA、rRNA 和 snoRNA 衍生的小 RNA 的表征及其对热应激的响应
DOI：
10.1371/journal.pone.0150933
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Yao Y
中科院分区：
文献类型：
--
作者：
Wang Y;Li H;Sun Q;Yao Y
从非编码RNA(NcRNAs)衍生的小RNAs(SRNAs)，如tRNAs、rRNAs和snoRNAs，已经在各种生物中被发现。一些观察表明，tRNAs和rRNAs的切割是由不同的胁迫诱导的。为了阐明小麦中来自tRNAs(StRNAs)、rRNAs(SrRNAs)和snoRNAs(SdRNAs)的sRNAs是否与热胁迫反应有关，我们对小麦幼苗的sRNA文库进行了生物信息学分析，并对对照和高温处理样品进行了比较，以测量stRNAs、srRNAs和sdRNAs的差异丰度。我们发现，tRNAs、5.8S rRNAs和28S rRNAs产生的sRNAs比5S rRNAs和18S rRNAs产生的sRNAs更具特异性，95%以上的stRNAs是从成熟tRNAs的3‘或5’端不对称加工而来的。我们鉴定出对热胁迫有反应的333个stRNA和8,822个srRNA。此外，来自tRNA-Val-CAC、tRNA-Thr-UgU、tRNA-Tyr-gua和tRNA-Ser-UGA的stRNAs的表达不仅在高温胁迫下上调，而且在渗透胁迫下也被诱导，这表明tRNAs裂解增加可能是小麦幼苗适应不利环境条件的一种机制。
Small RNAs (sRNAs) derived from non-coding RNAs (ncRNAs), such as tRNAs, rRNAs and snoRNAs, have been identified in various organisms. Several observations have indicated that cleavage of tRNAs and rRNAs is induced by various stresses. To clarify whether sRNAs in wheat derived from tRNAs (stRNAs), rRNAs (srRNAs) and snoRNAs (sdRNAs) are produced specifically in association with heat stress responses, we carried out a bioinformatic analysis of sRNA libraries from wheat seedlings and performed comparisons between control and high-temperature-treated samples to measure the differential abundance of stRNAs, srRNAs and sdRNAs. We found that the production of sRNAs from tRNAs, 5.8S rRNAs, and 28S rRNAs was more specific than that from 5S rRNAs and 18S rRNAs, and more than 95% of the stRNAs were processed asymmetrically from the 3’ or 5’ ends of mature tRNAs. We identified 333 stRNAs and 8,822 srRNAs that were responsive to heat stress. Moreover, the expression of stRNAs derived from tRNA-Val-CAC, tRNA-Thr-UGU, tRNA-Tyr-GUA and tRNA-Ser-UGA was not only up-regulated under heat stress but also induced by osmotic stress, suggesting that the increased cleavage of tRNAs might be a mechanism that developed in wheat seedlings to help them cope with adverse environmental conditions.