Mast cells accompany microvessels in human coronary atheromas: Implications for intimal neovascularization and hemorrhage
Mast cells accompany microvessels in human coronary atheromas: Implications for intimal neovascularization and hemorrhage
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DOI：
10.1016/0021-9150(95)05794-3
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发表时间：
1996-06-01
期刊：
影响因子：
5.3
通讯作者：
Kaartinen, M
中科院分区：
文献类型：
--
作者：
Kaartinen, M;Penttila, A;Kaartinen, M
肥大细胞被赋予了在新生血管中的作用。因此，我们检查了人类冠状动脉动脉粥样硬化的深层区域，也就是已知的容易发生新生血管的区域，以寻找肥大细胞的存在。用Elastica-van Gieson法和抗von Willebrand因子的单抗分别对37例年龄24~84岁的人冠状动脉内膜进行了染色，以检测新生血管的形成。用抗肥大细胞两种主要蛋白水解酶--类胰蛋白酶和糜乳酶的单抗对动脉粥样硬化进行染色，以检测肥大细胞。在发现的24个冠状动脉粥样斑块中，有13个深部含有肥大细胞。13个深部区域均可见新生血管，微血管周围的微血管数和肥大细胞数与动脉粥样硬化的大小密切相关。另一方面，在11个缺乏肥大细胞的深层区域中，只有一个区域显示了新生血管。在冠状动脉粥样斑块的新生血管区，肥大细胞与微血管紧密相连。所有的肥大细胞都含有类胰蛋白酶，其中一些含有凝乳酶，这两种酶都以其血管生成和基质降解能力而闻名。在光镜下观察到脱颗粒的肥大细胞，表明这些细胞被激活，并释放类胰蛋白酶和乳糜酶。含有血管生成因子的肥大细胞在人冠状动脉粥样硬化新生微血管周围的选择性定位表明，肥大细胞在这些病变的新生血管形成中发挥了作用。此外，肥大细胞还可能凭借其中性蛋白酶损伤微血管，从而产生斑块内出血，最终导致不稳定的损害。
Mast cells have been assigned a role in neovascularization. Therefore, we examined the deep regions of human coronary atheromas, the areas known to be prone to neovascularization, for the presence of mast cells. Specimens of atherosclerotic human coronary intima from 37 autopsy cases with ages of 24-84 pears were stained with elastica-van Gieson to detect atheroma formation and with monoclonal antibody against von Willebrand factor to detect neovascularization. Mast cells were detected by staining the atheromas with monoclonal antibodies against the two major proteases of mast cells, tryptase and chymase. Of the 24 coronary atheromas found, 13 contained mast cells in the deep regions. All these 13 deep regions also displayed neovascularization, and the number of microvessels and the number of mast cells around the microvessels correlated strongly with the size of the atheroma. On the other hand, of the 11 deep regions lacking mast cells, only one displayed neovascularization. In the neovascularized areas of the coronary atheromas, the mast cells were in close proximity to the microvessels. All the mast cells contained tryptase, and some of them chymase, both known for their angiogenic and matrix-degrading potential. In light microscopic studies, degranulated mast cells were observed indicating activation of these cells, with release of tryptase and chymase. The selective localization of activated mast cells containing angiogenic factors around newly formed microvessels in human coronary atheromas suggests that mast cells play a role in the neovascularization of these lesions. Moreover, mast cells may also, by virtue of their neutral proteases, injure the microvessels, and thereby produce intraplaque hemorrhages and, ultimately, unstable lesions.