Dental Pulp Stem Cell Migration
Dental Pulp Stem Cell Migration
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DOI：
10.1016/j.joen.2010.08.046
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发表时间：
2010-12-01
影响因子：
4.2
通讯作者：
Namerow, Kenneth N.
中科院分区：
文献类型：
--
作者：
Howard, Cameron;Murray, Peter E.;Namerow, Kenneth N.
目的：研究体外培养的牙髓干细胞(DPSCs)对化疗药物和细胞外基质蛋白(EMPs)的迁移作用。这种DPSC信号信息对于了解牙齿损伤后的再生和开发未来的再生性牙髓治疗方法是必要的。方法：用胰酶消化释放DPSCs，并将其植入Transwell滤膜。化学促进剂为重组鞘氨醇-1-磷酸(S1P)、成纤维细胞生长因子(FGF)、表皮生长因子(EGF)或转化生长因子-β1(TGF-β1)，EMP为胶原-1、胶原-IV、层粘连蛋白和纤维连接蛋白。数据采用方差分析(ANOVA)细胞迁移统计检验进行分析。结果：与其他转化生长因子-β1、成纤维细胞生长因子或表皮生长因子趋化因子相比，S1P诱导的DPSC迁移更为强烈(ANOVA，P<；0.05)。与其他EMP相比，层粘连蛋白诱导的DPSC迁移更活跃(ANOVA，P<；0.05)。结论：EMPs，特别是层粘连蛋白，以及化学活性物质，特别是S1P和转化生长因子-β1，被发现是DPSC迁移的重要促进剂。EMPs、血脂、血清和化学活性物质之间的相互作用表明DPSC的迁移受到高度调控。特异性的化学活性物质和EMPs可能参与了牙齿损伤后牙髓-牙本质的再生过程，可作为再生性牙髓治疗的一部分。(J Endod 2010；36：1963-1966)
Introduction: The purpose of this in vitro study was to investigate the migration of dental pulp stem cells (DPSCs) in response to chemotactants and extracellular matrix proteins (EMPs). This DPSC signaling information is needed to help understand tooth regeneration after injury and to develop some future regenerative endodontic therapies. Methods: DPSCs were released by trypsinization and plated on transwell filters. The chemotactants were recombinant sphingosine-1-phosphate (S1P), fibroblast growth factor (FGF), epidermal growth factor (EGF), or transforming growth factor beta-1 (TGF-beta 1), and the EMPs were collagen-1, collagen-IV, laminin, and fibronectin. Data were analyzed by using analysis of variance (ANOVA) statistical tests for cell migration. Results: S1P induced more vigorous DPSC migration in comparison with the other TGF- beta 1, FGF, or EFG chemotactants (ANOVA, P < .05). Laminin induced more vigorous DPSC migration in comparison with the other EMPs (ANOVA, P < .05). Conclusions: The EMPs, particularly laminin, and chemotactants, particularly S1P and TGF-beta 1, were found to be important promoters of DPSC migration. The interplay between the EMPs, blood lipid, serum, and chemotactants suggests that the migration of DPSC is highly regulated. Specific chemotactants and EMPs might mediate the process of pulp-dentin regeneration after tooth injury, and they could be used as part of regenerative endodontic therapy. (J Endod 2010;36:1963-1966)