Isolation and characterization of the major centipede allergen Sco m 5 from Scolopendra subspinipes mutilans
Isolation and characterization of the major centipede allergen Sco m 5 from Scolopendra subspinipes mutilans
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蜈蚣主要蜈蚣过敏原 Sco m 5 的分离和表征
DOI：
10.1016/j.alit.2020.06.003
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发表时间：
2021-01-01
影响因子：
6.8
通讯作者：
Lee, Wen-Hui
中科院分区：
文献类型：
--
作者：
Lan, Xin-Qiang;Zhao, Feng;Lee, Wen-Hui
背景资料：在蜈蚣直接叮咬和临床使用含有蜈蚣的药物（如利用蜈蚣的中药）后观察到过敏反应;然而，还没有天然蜈蚣过敏原的特征。S.采用Superdex 75凝胶过滤和RESOURCE S离子色谱法分离了一种Mutilans蛇毒，并结合LC-MS-MS、MALDI-TOF/TOF和蛋白质测序技术确定了其一级结构。结果：从S. S. Mutilans毒液Sco m 5能促进人肥大细胞系HMC-1的脱颗粒.在蜈蚣过敏患者中，Sco m 5显示83.3%的IgE结合率和66.7%的阳性反应率，分别通过免疫印迹和SPT检测。中国人群Sco m 5 IgE结合率为9%~ 16%。Sco m 5 IgE结合阳性血清与胡蜂毒有交叉反应。结论：本研究从中华胡蜂中分离并鉴定了一种新的变应原Sco m 5。S.残体。使用Sco m 5来识别蜈蚣过敏个体可能是重要的，因为Sco m 5在一般中国人群中具有高潜在的过敏原性，沿着蜈蚣过敏患者中对黄蜂毒液的交叉反应性的可能性。Copyright（C）2020，日本变态反应学会.由爱思唯尔公司制作和主持
Background: Allergic reactions have been observed following both direct centipede bites and the clinical use of centipede-containing medicines, such as traditional Chinese medicines utilizing Scolopendra subspinipes mutilans; however, no natural centipede allergen has yet been characterized.Methods: An allergen was purified from S. s. mutilans venom using Superdex 75 gel filtration and RESOURCE S ion chromatography, and its primary structure was determined via a combination of LC-MS-MS, MALDI-TOF/TOF and protein sequencing techniques. Its potential allergenicity was evaluated by immunoblotting, ELISAs, skin prick tests (SPTs) and mast cell activation assays.Results: A novel allergen Sco m 5 (210 amino acids long) was successfully purified from crude S. s. mutilans venom. Sco m 5 could promote the degranulation of a human mast cell line, HMC-1. Among centipede-allergic patients, Sco m 5 showed an 83.3% IgE-binding frequency and a 66.7% positive reaction frequency, as detected by immunoblotting and SPTs, respectively. Sco m 5 IgE-binding frequencies of common Chinese population was found to be 9%e16%. Sera positive for Sco m 5 IgE-binding was cross-reactive against venom from the wasp Vespa mandaeinia.Conclusions: The present study isolated and characterized a novel allergen termed as Sco m 5 from the centipede S. s. mutilans. The use of Sco m 5 to identify centipede-allergic individuals could be important, given the high potential allergenicity of Sco m 5 among the general Chinese population, along with the likely possibility of cross-reactivity against wasp venom among centipede-allergic patients. Copyright (C) 2020, Japanese Society of Allergology. Production and hosting by Elsevier B.V.