Limiting burn extension by transient inhibition of Connexin43 expression at the site of injury
Limiting burn extension by transient inhibition of Connexin43 expression at the site of injury
复制标题
DOI：
10.1016/j.bjps.2004.12.022
复制
发表时间：
2005-07-01
期刊：
影响因子：
--
通讯作者：
Becker, DL
中科院分区：
文献类型：
--
作者：
Coutinho, P;Qiu, C;Becker, DL
烧伤后24小时内创面的扩展是临床公认的，并导致诊断和治疗的困境。在中枢神经系统中，除了最初的损伤之外，类似的损伤扩散可以通过死亡信号从受损细胞通过连接蛋白43（Cx43）间隙连接通道传播到其健康的邻居而发生。在皮肤中，Cx43在基底表皮和成纤维细胞和真皮附属物中表达。我们已经使用Cx43特异性反义寡核苷酸的方法来短暂下调Cx43蛋白在部分厚度皮肤烧伤创面加热的早期阶段。反义ODN减少了组织损伤的扩散和损伤后伤口周围的中性粒细胞浸润。是形容词。与对照相比，细胞增殖增加，伤口闭合速度加快。与对照组相比，所得瘢痕较小，肉芽组织较少，真皮附属物较多。这些发现表明，Cx43反义治疗加速了部分厚度烧伤创面加热并减少了瘢痕形成。我们认为，这种方法可以提供一个有效的辅助管理部分厚度烧伤创面。(c)2005年，英国整形外科医生协会。由爱思唯尔有限公司出版。保留所有权利。
Extension of a burn wound over the first 24 h following injury is recognised clinically, and Leads to diagnostic and therapeutic dilemmas. In the central nervous system, a similar spread of damage, beyond the initial injury, can occur via the spread of death signals from injured cells to their healthy neighbours via Connexin43 (Cx43) gap junction channels. In the skin, Cx43 is expressed in the basal epidermis and in fibroblasts and dermal appendages. We have used Cx43 specific antisense oligodeoxynucleotide approach to transiently down-regulate Cx43 protein in the early stages of partial thickness cutaneous burn wound heating. Antisense ODNs reduce the spread of tissue damage and neutrophil infiltration around the wound following injury. Epithetial. cell proliferation is increased and the rate of wound closure is accelerated, compared to controls. Resultant scarring is smaller with less granulation tissue and more dermal appendages than controls. These findings suggest that Cx43 antisense treatment speeds partial thickness burn wound heating and reduces scarring. We suggest that this approach may provide an effective adjunct to managing partial thickness burn wounds. (c) 2005 The British Association of Plastic Surgeons. Published by Elsevier Ltd. All rights reserved.