Efficient open cultivation of cyanidialean red algae in acidified seawater
Efficient open cultivation of cyanidialean red algae in acidified seawater
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DOI：
10.1038/s41598-020-70398-z
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发表时间：
2020-08-24
影响因子：
4.6
通讯作者：
Miyagishima, Shin-ya
中科院分区：
文献类型：
--
作者：
Hirooka, Shunsuke;Tomita, Reiko;Miyagishima, Shin-ya
微藻在生产颜料、抗氧化剂和亲脂化合物方面具有很高的潜力，可用于工业应用。然而，他们的露天池塘养殖经常被其他不受欢迎的生物污染，包括他们的捕食者。此外，使用淡水的成本相对较高，与使用海水相比，这限制了养殖的地点和规模。此前研究表明，在含有相当于海水浓度的氯化钠的介质中，蓝藻和硫化银耳生长，而不是蓝藻。我们发现，在中等浓度的氯化钠存在下预培养的美罗兰能够使细胞在以海水为基础的培养基中生长。在以海水为基础的培养基中培养氰基红藻不需要额外的pH缓冲剂。此外，海水和酸性条件的结合比单独在酸性条件下更有效地降低了无菌开放培养的美罗罗非鱼受到其他生物污染的风险。
Microalgae possess high potential for producing pigments, antioxidants, and lipophilic compounds for industrial applications. However, their open pond cultures are often contaminated by other undesirable organisms, including their predators. In addition, the cost of using freshwater is relatively high, which limits the location and scale of cultivation compared with using seawater. It was previously shown that Cyanidium caldarium and Galdieria sulphuraria, but not Cyanidioschyzon merolae grew in media containing NaCl at a concentration equivalent to seawater. We found that the preculture of C. merolae in the presence of a moderate NaCl concentration enabled the cells to grow in the seawater-based medium. The cultivation of cyanidialean red algae in the seawater-based medium did not require additional pH buffering chemicals. In addition, the combination of seawater and acidic conditions reduced the risk of contamination by other organisms in the nonsterile open culture of C. merolae more efficiently than the acidic condition alone.