CX3CR1+ interstitial dendritic cells form a contiguous network throughout the entire kidney
CX3CR1+ interstitial dendritic cells form a contiguous network throughout the entire kidney
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DOI：
10.1038/sj.ki.5001567
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发表时间：
2006-08-01
影响因子：
19.6
通讯作者：
Nelson, P. J.
中科院分区：
文献类型：
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作者：
Soos, T. J.;Sims, T. N.;Nelson, P. J.
树突状细胞(DC)在非淋巴器官中起先天免疫和获得性免疫的作用；然而，DC在肾脏中的确切分布和类型尚不清楚。我们利用CX(3)CR1(GFP/+)小鼠来研究正常肾脏中CX(3)CR1(+)树突状细胞的解剖和表型。激光扫描共聚焦显微镜显示广泛的、连续的星状CX(3)CR1(+)树突状细胞网络遍布整个肾脏的间质和系膜间隙。浅层皮质活体显微镜显示静止的间质CX(3)CR1(+)树突状细胞，通过树突延伸持续探测周围组织环境。肾Cx(3)CR1(+)DC的流式细胞仪检测显示CD11c和F4/80显著共表达，主要组织相容性复合体II类和FCR高表达，未成熟的共刺激但有能力的吞噬能力表明组织驻留的未成熟DC准备对环境信号做出反应。因此，在肾实质内，肾Cx(3)CR1(+)DC提供的免疫豁免很少，Cx(3)CR1(+)DC是正常肾脏中异质单核吞噬细胞系统的主要组成部分。
Dendritic cells (DCs) interface innate and adaptive immunity in nonlymphoid organs; however, the exact distribution and types of DC within the kidney are not known. We utilized CX(3)CR1(GFP/+) mice to characterize the anatomy and phenotype of tissue-resident CX(3)CR1(+) DCs within normal kidney. Laser-scanning confocal microscopy revealed an extensive, contiguous network of stellate-shaped CX(3)CR1(+) DCs throughout the interstitial and mesangial spaces of the entire kidney. Intravital microscopy of the superficial cortex showed stationary interstitial CX(3)CR1(+) DCs that continually probe the surrounding tissue environment through dendrite extensions. Flow cytometry of renal CX(3)CR1(+) DCs showed significant coexpression of CD11c and F4/80, high major histocompatibility complex class II and FcR expression, and immature costimulatory but competent phagocytic ability indicative of tissue-resident, immature DCs ready to respond to environment cues. Thus, within the renal parenchyma, there exists little immunological privilege from the surveillance provided by renal CX(3)CR1(+) DCs, a major constituent of the heterogeneous mononuclear phagocyte system populating normal kidney.