IGHV1, IGHV5 and IGHV7 subgroup genes in the Rhesus macaque
IGHV1, IGHV5 and IGHV7 subgroup genes in the Rhesus macaque
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DOI：
10.1007/s00251-003-0536-2
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发表时间：
2003-03-01
期刊：
影响因子：
3.2
通讯作者：
Dunn-Walters, DK
中科院分区：
文献类型：
--
作者：
Bible, JM;Howard, W;Dunn-Walters, DK
抗体应答的多样性部分是通过不同免疫球蛋白（IG）基因的重排实现的。IG重链由可变区（IGHV）、多样性区（IGHD）和连接区（IGHJ）组成。人生殖系IGHV基因已被分为七个多基因亚组。这些亚组的大小和使用情况不等，IGHV 3亚组最常用（36%），其次是IGHV 1/7（26%），然后是IGHV 4、IGHV 5、IGHV 2、IGHV 6（分别为15%、12%、4%、3%）。恒河猴（Macaca mulatta）是研究感染的一种有用的非人灵长类动物模型，猕猴的生殖系IG基因数据库正逐渐发展成为研究B细胞反应的有用工具。猕猴中IGHV亚群的使用比例与人类相似。猕猴IGHV 3和IGHV 4亚群的代表已被发表，IGHD和IGHJ基因的种系序列也已被发表。然而，迄今为止，还没有发表来自第二大IGHV亚组IGHV 1的序列。我们报告的分离和测序的基因组片段含有IGHV 1基因的猕猴。根据该序列设计的聚合酶链反应（PCR）引物使我们能够扩增和测序25个新的IGHV 1生殖系基因。我们还分离了两个IGHV 7基因，使用相同的引物，和两个IGHV 5基因，使用人IGHV 5引物。
The diversity of the antibody response is achieved, in part, by rearrangement of different immunoglobulin (Ig) genes. The Ig heavy chain is made up of a variable region (IGHV), a diversity region (IGHD) and a joining region (IGHJ). Human germline IGHV genes have been grouped into seven multigene subgroups. Size and usage of these subgroups is not equal, the IGHV3 subgroup is the most commonly used (36%), followed by IGHV1/7 (26%), then IGHV4, IGHV5, IGHV2, IGHV6 (15%, 12%, 4%, 3% respectively). The rhesus macaque (Macaca mulatta) is a useful non-human primate model for studies of infection and the database of germline Ig genes for the macaque is gradually growing to become a useful tool in the study of B-cell responses. The proportions of IGHV subgroup usage in the macaque are similar to those in man. Representatives from IGHV3 and IGHV4 subgroups for the macaque have been published, as have germline sequences of the IGHD and IGHJ genes. However, to date there have been no sequences published from the second largest IGHV subgroup, IGHV1. We report the isolation and sequencing of a genomic fragment containing an IGHV1 gene from the macaque. Polymerase chain reaction (PCR) primers designed from this sequence enabled us to amplify and sequence 25 new IGHV1 germline genes. We also isolated two IGHV7 genes, using the same primers, and two IGHV5 genes, using human IGHV5 primers.