Monitoring of spectroscopic changes of a single trapped fission yeast cell by using a Raman tweezers set-up
Monitoring of spectroscopic changes of a single trapped fission yeast cell by using a Raman tweezers set-up
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DOI：
10.1016/j.optcom.2008.06.074
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发表时间：
2008-10
影响因子：
2.4
通讯作者：
G. Başar;S. Kin
中科院分区：
文献类型：
--
作者：
G. Başar;S. Kin
We demonstrate an improvement of the sensitivity of a Raman tweezers set-up, which combines optical tweezers with Raman spectroscopy. The system was tested by taking the Raman spectrum of a 4.6μm diameter polystyrene sphere trapped in an aqueous solution. The improvement of sensitivity of the set-up was achieved by adjusting the trap depth for maximum signal to noise ratio (SNR). The maximum SNR was obtained by investigating the Raman peak of a trapped polystyrene sphere at 1001cm−1according to trap depth. With this system, a single trapped living Schizosaccharomyces Pombe yeast cell was sensitively monitored by taking the kinetic Raman spectra for more than 2h. The relative intensity decrease in amide I and amide III bands, frequency increase in amide I band together with alterations in tyrosine marker band around 850cm−1was observed, which indicates alterations in the hydration state of protein by time progressing.
