Requirement for p53 and p21 to sustain G2 arrest after DNA damage
Requirement for p53 and p21 to sustain G2 arrest after DNA damage
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DOI：
10.1126/science.282.5393.1497
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发表时间：
1998-11-20
期刊：
影响因子：
56.9
通讯作者：
Vogelstein, B
中科院分区：
文献类型：
--
作者：
Bunz, F;Dutriaux, A;Vogelstein, B
在DNA损伤后，许多细胞似乎进入细胞周期的G（2）期的持续停滞。它在这里表明，这种逮捕可以持续只有当p53存在于细胞中，并能够转录激活细胞周期蛋白依赖性激酶抑制剂p21。在p53或p21基因被破坏后，γ射线照射的细胞进入有丝分裂，并仅由于胞质分裂失败而显示出G（2）DNA含量。因此，p53和p21似乎是维持人类细胞中G（2）检查点所必需的。
After DNA damage, many cells appear to enter a sustained arrest in the G(2) phase of the cell cycle. It is shown here that this arrest could be sustained only when p53 was present in the cell and capable of transcriptionally activating the cyclin-dependent kinase inhibitor p21. After disruption of either the p53 or the p21 gene, gamma radiated cells progressed into mitosis and exhibited a G(2) DNA content only because of a failure of cytokinesis. Thus, p53 and p21 appear to be essential for maintaining the G(2) checkpoint in human cells.