Insights into the adaptive response of the plant-pathogenic oomycete Phytophthora capsici to the fungicide flumorph.
Insights into the adaptive response of the plant-pathogenic oomycete Phytophthora capsici to the fungicide flumorph.
复制标题
深入了解植物致病卵菌辣椒疫霉对杀菌剂氟吗啉的适应性反应
DOI：
10.1038/srep24103
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发表时间：
2016-04-06
影响因子：
4.6
通讯作者：
Liu X
中科院分区：
文献类型：
--
作者：
Pang Z;Chen L;Mu W;Liu L;Liu X
辣椒疫霉是一种重要的卵菌植物病原菌，在世界范围内造成重大损失。羧酸酰胺类杀菌剂氟吗啉对卵菌植物病原体表现出优异的活性。尽管它的潜力，仍然令人担忧的是，卵菌病原体的有性繁殖，导致基因重组，可能会导致快速发展的耐药性氟吗啉。本研究利用基于iTRAQ（isobaric tags for relative and absolute quantitation）的方法来比较亲本辣椒疫霉分离物PCAS 1及其有性后代S2-838的蛋白质组之间的差异，其表现出对氟吗啉的显著抗性。共鉴定了2396个蛋白质，其中181个蛋白质被认为与辣椒疫霉对氟吗啉的适应性反应有关。随后的生物信息学分析表明，辣椒疫霉对氟吗啉的适应性反应是复杂的，并受到多种机制的调控，包括利用宿主环境中的碳水化合物来补偿氟吗啉诱导的细胞壁应激，能量产生的转变，氨基酸生物合成的减少，以及与病原体对刺激和跨膜运输的反应相关的蛋白质水平的升高。研究结果为辣椒疫霉田间种群对氟吗啉抗性的早期监测提供了重要依据。
Phytophthora capsici is an important oomycete plant pathogen that causes significant losses worldwide. The carboxylic acid amide fungicide flumorph has shown excellent activity against oomycete plant pathogens. Despite its potential, there remains concern that the sexual reproduction of oomycete pathogens, which results in genetic recombination, could result in the rapid development of resistance to flumorph. The current study utilized an iTRAQ (isobaric tags for relative and absolute quantitation) based method to compare differences between the proteome of the parental P. capsici isolate PCAS1 and its sexual progeny S2-838, which exhibits significant resistance to flumorph. A total of 2396 individual proteins were identified, of these, 181 were considered to be associated with the adaptive response of P. capsici to flumorph. The subsequent bioinformatic analysis revealed that the adaptive response of P. capsici to flumorph was complex and regulated by multiple mechanisms, including utilising carbohydrate from the host environment to compensate for the cell wall stress induced by flumorph, a shift in energy generation, decreased amino acids biosynthesis, and elevated levels of proteins associated with the pathogen’s response to stimulus and transmembrane transport. Moreover, the results of the study provided crucial data that could provide the basis for early monitoring of flumorph resistance in field populations of P. capsici.