MAEBL is essential for malarial sporozoite infection of the mosquito salivary gland
MAEBL is essential for malarial sporozoite infection of the mosquito salivary gland
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DOI：
10.1084/jem.20011876
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发表时间：
2002-05-20
影响因子：
15.3
通讯作者：
Chinzei, Y
中科院分区：
文献类型：
--
作者：
Kariu, T;Yuda, M;Chinzei, Y
疟疾的子孢子在蚊子中肠壁形成的卵囊中成熟，然后选择性地侵入唾液腺，在那里它们等待通过蚊子叮咬传播给脊椎动物宿主。唾液腺的侵袭一直被认为是由特定的配体-受体相互作用所介导的，但参与这些相互作用的分子仍不清楚。MAEBL是一种结构上与裂殖子黏附蛋白相关的单一跨膜样蛋白。我们发现啮齿动物疟原虫北极星的MA_EBL是由卵囊内的子孢子产生的，并定位于其微线体中，微线体是参与疟疾寄生虫入侵宿主细胞的分泌细胞器。伯氏假单胞菌MAEBL基因的靶向破坏实验表明，MAEBL基因是唾液腺子孢子感染所必需的，并参与了唾液腺表面的附着。相反，MAEBL基因的破坏并不影响子孢子在体外的运动能力，也不影响对脊椎动物宿主的感染性。这些结果表明，白纹伊蚊MAEBL是一种子孢子附着蛋白，参与与蚊子唾液腺的特异性结合和感染。
Malarial sporozoites mature in the oocysts formed in the mosquito midgut wall and then selectively invade the salivary glands, where they wait to be transmitted to the vertebrate host via mosquito bite. Invasion into the salivary gland has been thought to be mediated by specific ligand-receptor interactions, but the molecules involved in these interactions remain unknown. MAEBL is a single transmembrane-like protein that is structurally related to merozoite adhesive proteins. We found MA_EBL of the rodent malaria parasite, Plasazodiiiin beigizei, to be specificaEy produced by the sporozoites in the oocyst and localized in their micronemes, which are secretory organelles involved in malarial parasite invasion into the host cell. A targeted disruption experiment of the P. berghei MAEBL gene revealed that it was essential for sporozoite infection of the salivary gland and was involved in the attachment to the salivary gland surface. In contrast, the disruption of the MAEBL gene did not affect sporozoite motility in vitro nor infectivity to the vertebrate host. These results suggest that P. beighei MAEBL is a sporozoite attachment protein that participates in specific binding to and infection of the mosquito salivary gland.