Harnessing the Angiogenic Potential of Stem Cell-Derived Exosomes for Vascular Regeneration.
Harnessing the Angiogenic Potential of Stem Cell-Derived Exosomes for Vascular Regeneration.
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利用干细胞衍生的外泌体的血管生成潜力用于血管再生。
DOI：
10.1155/2016/3409169
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发表时间：
2016
影响因子：
4.3
通讯作者：
Khoury M
中科院分区：
文献类型：
--
作者：
Alcayaga-Miranda F;Varas-Godoy M;Khoury M
间充质干细胞（MSC）是已知的显示重要的再生性能，通过分泌促血管生成因子。最近的证据指出，从MSC释放的外泌体在这种旁分泌机制中发挥着关键作用。外泌体是细胞间通讯的关键介质，并含有包括可修饰的microRNA（miRNA）、mRNA和蛋白质内容物的货物。由于MSC衍生的外泌体的生物发生受MSC与其生态位之间的串扰调节，因此外泌体的含量以及因此其生物学功能取决于来源的细胞及其微环境的生理或病理状态。最近的临床前研究表明，MSC衍生的外泌体在血管生成过程中具有重要意义，因为使用外泌体耗尽的条件培养基损害了MSC血管生成反应。在这篇综述中，我们讨论了目前的知识有关的血管生成潜力的MSC外泌体和方法，以提高其生物活性，改善血管再生。目前对外来体研究的深入了解突出了在治疗性血管生成中组合基于细胞的疗法及其分泌产物的能力。
Mesenchymal stem cells (MSCs) are known to display important regenerative properties through the secretion of proangiogenic factors. Recent evidence pointed at the key role played by exosomes released from MSCs in this paracrine mechanism. Exosomes are key mediators of intercellular communication and contain a cargo that includes a modifiable content of microRNA (miRNA), mRNA, and proteins. Since the biogenesis of the MSCs-derived exosomes is regulated by the cross talk between MSCs and their niche, the content of the exosomes and consequently their biological function are dependent on the cell of origin and the physiologic or pathologic status of their microenvironment. Recent preclinical studies revealed that MSCs-derived exosomes have a critical implication in the angiogenic process since the use of exosomes-depleted conditioned medium impaired the MSCs angiogenesis response. In this review, we discuss the current knowledge related to the angiogenic potential of MSCs-exosomes and methods to enhance their biological activities for improved vascular regeneration. The current gain of insight in exosomes studies highlights the power of combining cell based therapies and their secreted products in therapeutic angiogenesis.