Comparison of radioimmunoassay and enzyme-linked immunoassay methods for detection of Histoplasma capsulatum var. capsulatum antigen
Comparison of radioimmunoassay and enzyme-linked immunoassay methods for detection of Histoplasma capsulatum var. capsulatum antigen
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DOI：
10.1128/jcm.35.9.2252-2255.1997
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发表时间：
1997-09-01
影响因子：
9.4
通讯作者：
Wheat, LJ
中科院分区：
文献类型：
--
作者：
Durkin, MM;Connolly, PA;Wheat, LJ
本研究的目的是比较传统的放射免疫测定法（RIA）和酶联免疫测定法（EIA）对储存尿液标本中组织浆菌抗原的测量，两种方法之间的相关性将允许EIA作为已建立的I-125 RIA的非放射性替代方法。该研究使用了1988年在印第安纳波利斯爆发的组织浆菌病期间诊断为患者的尿液，对照标本来自健康成人。其他真菌感染、尿路感染或非真菌肺炎患者也进行检测，RIA和EIA同时进行，RIA系统测量抗原水平为0.4 ~ 27.0 RIA单位，ETA测量抗原水平为0.6 ~ 20.1单位。56例弥散性疾病患者中有50例（89%），30例自限性疾病患者中有11例（37%），EIA和RU均检测出尿液中可测量的抗原水平。来自副球孢子菌病患者的96例对照标本中有1例在两种系统中均呈阳性。EIA测定的抗原水平与RIA测定的抗原水平具有良好的相关性（相关系数为0.974），EIA是RIA测定尿液标本中组织浆抗原水平的可接受替代方法。
The purpose of this study was to compare the conventional radioimmunoassay (RIA) to an enzyme-linked immunoassay (EIA) far the measurement of Histoplasma antigen in banked urine specimens, A correlation between the two methods would at allow the EIA to be used as a nonradioactive alternative to the established I-125 RIA, The study used stored urine from patients diagnosed with histoplasmosis during an outbreak in Indianapolis which began in 1988, Control specimens from healthy adults, patients with other fungal infections, urinary tract infections, or nonfungal pneumonia were also tested, Both the RIA and EIA were run concurrently, The RIA system measured antigen levels of 0.4 to 27.0 RIA units, while the ETA measured antigen levels of 0.6 to 20.1 units. Both the EIA and RU defected measurable antigen levels in urine from 50 of 56 patients (89%) with disseminated disease and 11 of 30 patients (37%) with self-limiting disease, One of 96 control specimens, from a patient with paracoccidioidomycosis, was positive viith both systems. Antigen levels measured by EIA correlated well with those measured by the established RIA method (correlation coefficient, 0.974), The EIA is an acceptable alternative to the RIA for measuring Histoplasma antigen levels in urine specimens.