Blocking P2X receptors can inhibit the injury-induced proliferation of olfactory epithelium progenitor cells in adult mouse
Blocking P2X receptors can inhibit the injury-induced proliferation of olfactory epithelium progenitor cells in adult mouse
复制标题
阻断 P2X 受体可以抑制成年小鼠损伤诱导的嗅上皮祖细胞增殖。
DOI：
10.1016/j.ijporl.2010.03.030
复制
发表时间：
2010-07-01
影响因子：
1.5
通讯作者：
He, Cheng
中科院分区：
文献类型：
--
作者：
Gao, Liang;Cao, Li;He, Cheng
目的：嗅上皮（OE）具有显著的再生能力和在成年期持续发生的嗅受体神经元（orn）。骨性器官的再生是由骨性器官中的基底细胞完成的，包括干细胞和祖细胞。虽然对OE基底细胞在骨髓瘤细胞更新中的作用有相当多的了解，但调节成年OE基底细胞增殖和分化的分子机制尚不完全清楚。嘌呤作为细胞间信号分子，已被报道参与多种神经干细胞的增殖、分化和迁移。然而，损伤的骨膜组织释放的ATP是否在骨膜组织转换中调控神经发生尚不清楚。方法：采用RT-PCR和免疫组织化学方法检测成年小鼠OE中嗜电性嘌呤能受体- p2x受体的表达。采用嗅球切除模型和体内给药P2X受体拮抗剂，研究P2X受体对OE祖细胞增殖的调控作用。结果：我们发现成年小鼠OE基底细胞表达功能性P2X受体，阻断P2X受体活性可显著抑制OE基底细胞损伤诱导的增殖。结论：本研究支持嘌呤能信号作为旁分泌信号调控成年小鼠OE神经发生的假说。2010爱思唯尔爱尔兰有限公司版权所有。
Objective: The olfactory epithelium (OE) is unusual for its remarkable regenerative capacity and sustained neurogenesis of olfactory receptor neurons (ORNs) throughout adult life. Regeneration of ORNs is accomplished by basal cells in the OE, including stem cells and progenitor cells. Although there is considerable knowledge about the roles of OE basal cells in URN turnover, the molecular mechanism that regulates the proliferation and differentiation of adult OE basal cells is not fully understood. As intercellular signaling molecules, purines have been reported to meditate proliferation, differentiation and migration of many kinds of neural stem cells. However, it is still unclear whether ATP, which could be released by injured ORNs, plays a role in regulating neurogenesis in URN turnover.Methods: RT-PCR and immunohistochemistry were used to detect the expression of ionotropic purinergic receptors-P2X receptors in adult mouse OE. By using the olfactory bulbectomy model and in vivo administration of P2X receptors antagonists, the function of P2X receptors in regulating the proliferation of OE progenitor cell was evaluated.Results: We found that basal cells in the adult mouse OE express functional P2X receptors, and blocking the activities of P2X receptors can significantly inhibit the injury-induced proliferation of OE basal cells.Conclusion: Our research provides evidence in support of the hypothesis that purinergic signaling can serve as a paracrine signal in regulating the neurogenesis of OE in adult mouse. (C) 2010 Elsevier Ireland Ltd. All rights reserved.