Bacterial diversity in typical abandoned multi-contaminated nonferrous metal(loid) tailings during natural attenuation
Bacterial diversity in typical abandoned multi-contaminated nonferrous metal(loid) tailings during natural attenuation
复制标题
典型废弃多污染有色金属尾矿自然衰减过程中的细菌多样性
DOI：
10.1016/j.envpol.2018.12.045
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发表时间：
2019-04-01
影响因子：
8.9
通讯作者：
Alakangas, Lena
中科院分区：
文献类型：
--
作者：
Liu, Jian-li;Yao, Jun;Alakangas, Lena
废弃的有色金属尾矿场地是人为的，代表了微生物群落独特和极端的生态位。尾矿中金属（样物质）含量偏高且有毒，对当地人类健康和区域生态系统造成负面影响。在这些经过自然衰减的典型尾矿中，微生物群落往往很少得到检查。本文对广西7个有色金属尾矿库进行了细菌群落多样性和功能分析，这些尾矿库在3 ~ 31年前被废弃。尾矿场的酸度在31年的不活动期间有所上升。在废弃3年的尾矿中，脱硫弧菌一直与硫/硫化物氧化相结合，对硝酸盐/亚硝酸盐进行异化还原。而对植物生长有利的根瘤菌属（Rhizobium）、铁/硫氧化菌属（Acidiferrobacter）和酸性硫杆菌属（Acidithiobacillus）在23年以上的废弃尾矿中具有特异性。在自然衰减过程中，产酸铁/硫氧化菌和金属（类）相关菌及特定菌群丰度的增加，为了解尾矿微生物生态系统功能提供了新的思路。与硫杆菌属、芽孢杆菌属、硫杆菌属、盖氏菌属和硫杆菌属相关的OTUs是区分不同尾矿点细菌群落的主要贡献者。细菌群落与地球化学参数的多重相关分析表明，pH、TOC、TN、As、Pb和Cu是影响细菌群落结构的主要驱动因素。PICRUSt功能探索发现其主要功能与DNA修复和重组有关，是细菌适应尾矿多重污染的重要功能。这些信息为指导未来的宏基因组研究提供了新的见解，以确定金属转化/抗性以外的关键功能。此外，我们的研究结果为多重污染的有色金属尾矿场地自然衰减过程中细菌群落的管理提供了新的前景。爱思唯尔2019年版权所有版权所有。
Abandoned nonferrous metal(loid) tailings sites are anthropogenic, and represent unique and extreme ecological niches for microbial communities. Tailings contain elevated and toxic content of metal(loid)s that had negative effects on local human health and regional ecosystems. Microbial communities in these typical tailings undergoing natural attenuation are often very poorly examined. The diversity and inferred functions of bacterial communities were examined at seven nonferrous metal(loid) tailings sites in Guangxi (China), which were abandoned between 3 and 31 years ago. The acidity of the tailings sites rose over 31 years of site inactivity. Desulfurivibrio, which were always coupled with sulfur/sulfide oxidation to dissimilate the reduction of nitrate/nitrite, were specific in tailings with 3 years abandonment. However, genus beneficial to plant growth (Rhizobium), and iron/sulfur-oxidizing bacteria and metal(loid)-related genera (Acidiferrobacter and Acidithiobacillus) were specific within tailings abandoned for 23 years or more. The increased abundance of acid-generating iron/sulfur-oxidizing and metal(loid)-related bacteria and specific bacterial communities during the natural attenuation could provide new insights for understanding microbial ecosystem functioning in mine tailings. OTUs related to Sulfuriferula, Bacillus, Sulfurifustis, Gaiella, and Thiobacillus genera were the main contributors differentiating the bacterial communities between the different tailing sites. Multiple correlation analyses between bacterial communities and geochemical parameters indicated that pH, TOC, TN, As, Pb, and Cu were the main drivers influencing the bacterial community structures. PICRUSt functional exploration revealed that the main functions were related to DNA repair and recombination, important functions for bacterial adaptation to cope with the multi-contamination of tailings. Such information provides new insights to guide future metagenomic studies for the identification of key functions beyond metal-transformation/resistance. As well, our results offers novel outlooks for the management of bacterial communities during natural attenuation of multi-contaminated nonferrous metal(loid) tailings sites. Crown Copyright (C) 2019 Published by Elsevier Ltd. All rights reserved.