Structural studies of SSB interaction with RecO.
Structural studies of SSB interaction with RecO.
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DOI：
10.1007/978-1-62703-032-8_7
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发表时间：
2012
影响因子：
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通讯作者：
Mikhail Ryzhikov;S. Korolev
中科院分区：
文献类型：
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作者：
Mikhail Ryzhikov;S. Korolev
重组蛋白RecO与单链（ss）DNA结合蛋白（SSB）的相互作用对于DNA损伤修复和停滞复制的重新启动是必需的（考克斯，生物化学分子生物学评论（Crit Rev Biochem Mol Biol）42（1）：41-63，2007）。为了了解这种相互作用的机制及其在DNA修复中的作用，我们破译了一个具有SSB C-末端尾的RecO复合物（SSB-Ct）的高分辨率结构。该结构揭示了两种蛋白质之间的疏水相互作用的关键作用，并表明在同源重组和链退火过程中RecO募集到DNA的机制。
Interaction of recombination protein RecO with single-stranded (ss) DNA-binding protein (SSB) is essential for DNA damage repair and restart of stalled replication (Cox, Crit Rev Biochem Mol Biol 42(1):41–63, 2007). To understand mechanism of this interaction and its role in DNA repair, we deciphered a high-resolution structure of RecO complex with C-terminal tail of SSB (SSB-Ct). The structure revealed a key role of hydrophobic interactions between two proteins and suggests the mechanism of RecO recruitment to DNA during homologous recombination and strand annealing.