Transcriptional Activities of the Microbial Consortium Living with the Marine Nitrogen-Fixing Cyanobacterium Trichodesmium Reveal Potential Roles in Community-Level Nitrogen Cycling
Transcriptional Activities of the Microbial Consortium Living with the Marine Nitrogen-Fixing Cyanobacterium Trichodesmium Reveal Potential Roles in Community-Level Nitrogen Cycling
复制标题
与海洋固氮蓝藻Trichodesmium一起生活的微生物群落的转录活性揭示了群落水平氮循环的潜在作用
DOI：
10.1128/aem.02026-17
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发表时间：
2017
影响因子：
4.4
通讯作者：
Drake, Harold L.
中科院分区：
文献类型：
--
作者：
Lee, Michael D.;Webb, Eric A.;Walworth, Nathan G.;Fu, Fei-Xue;Held, Noelle A.;Saito, Mak A.;Hutchins, David A.;Drake, Harold L.
束毛藻是一种分布于全球的蓝细菌，其固氮能力在温暖的贫营养海洋中为初级生产提供燃料。像许多光合自养生物一样，束毛藻是各种其他微生物的宿主，但人们对这种相关群落如何调节环境相关化学物质进出超生物体结构的通量知之甚少。在这里，我们利用元转录组学来研究与Trichodesmium pyrraeum（菌株IMS 101）相关的微生物群落的基因表达活性，使用实验室维持的富集培养物，这些富集培养物先前已被证明具有与自然种群相似的微生物群落。在两种不同的CO2浓度下保持了108年的富集中，发现群落转录谱对处理具有特异性，表明发生了整体基因表达的重组。这种结构调整涉及的一些显着增加，在CO2浓度升高的社区呼吸相关的成绩单，有可能促进相应的测量主机固氮率的增加。特别值得注意的是，在这两种处理中，检测到参与硝酸盐，亚硝酸盐和一氧化二氮还原的群落转录物，这表明相关的生物体可能在菌落水平的氮循环中发挥作用。最后，分类特异性分析显示，不同的生态位一贯共存的主要类群，可能使，甚至鼓励，稳定的不同社区内的同居Trichodesmium consortia.IMPORTANCETrichodesmium是一个属的全球分布，固氮海洋蓝藻。作为氮缺乏系统中新氮的来源，这些生物有助于燃料碳固定，由其他更丰富的光合自养生物进行，从而在全球氮和碳循环中发挥重要作用。束毛藻属的成员倾向于形成大型的宏观菌落，这些菌落似乎永久地承载着与周围海水不同的多种相互作用的微生物的协会，可能使整个组合成为一个独特的微型生态系统。自20世纪90年代初首次成功栽培以来，一直有人质疑束毛藻及其相关微生物群落之间的潜在相互依赖性，以及宿主看似神秘的固氮模式是否以某种方式涉及或受益于其上生生物。在这里，我们用新技术重新审视这些老问题，并研究与束毛菌相关的微生物群落的基因表达活性。
Trichodesmium is a globally distributed cyanobacterium whose nitrogen-fixing capability fuels primary production in warm oligotrophic oceans. Like many photoautotrophs, Trichodesmium serves as a host to various other microorganisms, yet little is known about how this associated community modulates fluxes of environmentally relevant chemical species into and out of the supraorganismal structure. Here, we utilized metatranscriptomics to examine gene expression activities of microbial communities associated with Trichodesmium erythraeum (strain IMS101) using laboratory-maintained enrichment cultures that have previously been shown to harbor microbial communities similar to those of natural populations. In enrichments maintained under two distinct CO2concentrations for ∼8 years, the community transcriptional profiles were found to be specific to the treatment, demonstrating a restructuring of overall gene expression had occurred. Some of this restructuring involved significant increases in community respiration-related transcripts under elevated CO2, potentially facilitating the corresponding measured increases in host nitrogen fixation rates. Particularly of note, in both treatments, community transcripts involved in the reduction of nitrate, nitrite, and nitrous oxide were detected, suggesting the associated organisms may play a role in colony-level nitrogen cycling. Lastly, a taxon-specific analysis revealed distinct ecological niches of consistently cooccurring major taxa that may enable, or even encourage, the stable cohabitation of a diverse community within Trichodesmium consortia.IMPORTANCETrichodesmium is a genus of globally distributed, nitrogen-fixing marine cyanobacteria. As a source of new nitrogen in otherwise nitrogen-deficient systems, these organisms help fuel carbon fixation carried out by other more abundant photoautotrophs and thereby have significant roles in global nitrogen and carbon cycling. Members of the Trichodesmium genus tend to form large macroscopic colonies that appear to perpetually host an association of diverse interacting microbes distinct from the surrounding seawater, potentially making the entire assemblage a unique miniature ecosystem. Since its first successful cultivation in the early 1990s, there have been questions about the potential interdependencies between Trichodesmium and its associated microbial community and whether the host's seemingly enigmatic nitrogen fixation schema somehow involved or benefited from its epibionts. Here, we revisit these old questions with new technology and investigate gene expression activities of microbial communities living in association with Trichodesmium.
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海洋浮游蓝藻 Trichodesmium 的丝状蓝藻伙伴
DOI：
--
发表时间：
1992
期刊：
影响因子：
--
作者：
P. J. Siddiqui;B. Bergman;E. Carpenter
通讯作者：
E. Carpenter
细菌-鱼腥藻（蓝藻科）关联对于淡水中 N2 固定的意义 1, 2
影响因子：
2.9
作者：
H. Paerl;P. Kellar
通讯作者：
P. Kellar
与海洋振荡菌 SP 的细菌关联。
DOI：
--
发表时间：
1989
期刊：
影响因子：
--
作者：
Hans W. Paerly;B. Bebout;L. Prufert
通讯作者：
L. Prufert
影响因子：
11
作者：
Momper, Lily M.;Reese, Brandi Kiel;Webb, Eric A.
通讯作者：
Webb, Eric A.
影响因子：
56.9
作者：
CARPENTER, EJ;PRICE, CC
通讯作者：
PRICE, CC