Evaluation of Methods of Determining Humic Acids in Nucleic Acid Samples for Molecular Biological Analysis
Evaluation of Methods of Determining Humic Acids in Nucleic Acid Samples for Molecular Biological Analysis
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DOI：
10.1271/bbb.100597
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发表时间：
2011-02
期刊：
影响因子：
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通讯作者：
Yong Wang;T. Fujii
中科院分区：
文献类型：
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作者：
Yong Wang;T. Fujii
在分子生物学分析中，评估从土壤中提取的DNA/RNA中共提取的腐植酸的污染是很重要的。我们比较了各种腐植酸测定方法的灵敏度，以及DNA/RNA和蛋白质对测定的影响。结合实验结果，对分子生物学分析中腐植酸测定方法的选择提出了建议。
It is important in molecular biological analyses to evaluate contamination of co-extracted humic acids in DNA/RNA extracted from soil. We compared the sensitivity of various methods for measurement of humic acids, and influences of DNA/RNA and proteins on the measurement. Considering the results, we give suggestions as to choice of methods for measurement of humic acids in molecular biological analyses.