The use of fluorescent proteins for intravital imaging of cancer cell invasion.
The use of fluorescent proteins for intravital imaging of cancer cell invasion.
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DOI：
10.1007/978-1-61779-797-2_2
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发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Segall, Jeffrey E
中科院分区：
文献类型：
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作者：
Hulit, James;Kedrin, Dmitriy;Segall, Jeffrey E
对活体动物原发肿瘤中癌细胞行为的分析为探索体内存在的复杂微环境中的侵袭和内渗过程提供了机会。在本章中，我们描述了我们为乳腺肿瘤进行活体成像而开发的方法。我们提供通过注射肿瘤细胞系产生肿瘤的程序，以及使用皮瓣肿瘤解剖和乳腺成像窗口进行多光子成像。
The analysis of cancer cell behavior in the primary tumor in living animals provides an opportunity to explore the process of invasion and intravasation in the complex microenvironment that is present in vivo. In this chapter, we describe the methods that we have developed for performing intravital imaging of mammary tumors. We provide procedures for generating tumors through injection of tumor cell lines, and multiphoton imaging using a skin-flap tumor dissection and a mammary imaging window.