The mechanism of axl-mediated Ebola virus infection
The mechanism of axl-mediated Ebola virus infection
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DOI：
10.1086/520594
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发表时间：
2007-11-15
影响因子：
6.4
通讯作者：
Kawaoka, Yoshihiro
中科院分区：
文献类型：
--
作者：
Shimojima, Masayuki;Ikeda, Yasuhiro;Kawaoka, Yoshihiro
我们之前报道了受体型酪氨酸激酶Axl的表达，它调节细胞存活和激活，增强假埃博拉病毒和活埃博拉病毒（EBOV）感染。为了阐明这种增强的机制基础，我们利用携带EBOV糖蛋白（GP）的伪病毒创建了一系列Axl突变体，并鉴定了该功能所需的氨基酸/结构域。对Axl突变体的分析表明，在EBOV gp介导的感染中，细胞外和细胞内区域对Axl功能的重要性，包括配体结合和信号转导。这些数据表明EBOV利用Axl的生理功能进入细胞。
We previously reported that expression of the receptor-type tyrosine kinase Axl, which regulates cell survival and activation, enhances both pseudotype and live Ebola virus (EBOV) infection. To clarify the mechanistic basis of this enhancement, we created a series of Axl mutants and identified amino acids/domains necessary for this function, by using a pseudotype virus carrying the EBOV glycoprotein (GP). Analyses of the Axl mutants showed the importance of extracellular and intracellular regions for Axl functions, including ligand binding and signal transduction, in EBOV GP-mediated infection. These data suggest that EBOV uses the physiological functions of Axl to enter cells.