THE ENDOPARASITOID Pteromalus puparum INFLUENCES HOST GENE EXPRESSION WITHIN FIRST HOUR OF PARASITIZATION.
THE ENDOPARASITOID Pteromalus puparum INFLUENCES HOST GENE EXPRESSION WITHIN FIRST HOUR OF PARASITIZATION.
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DOI：
10.1002/arch.21250
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发表时间：
2015-11
影响因子：
2.2
通讯作者：
Yu Zhu;Qi Fang;Yang Liu;Lingfeng Gao;Zhichao Yan;G. Yè
中科院分区：
文献类型：
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作者：
Yu Zhu;Qi Fang;Yang Liu;Lingfeng Gao;Zhichao Yan;G. Yè
小菜粉蝶Pieris rapae是十字花科植物的重要害虫，Pteromalus puparum是该蝴蝶的优势蛹内寄生蜂。为了成功发育寄生蜂后代，雌性寄生蜂通常在产卵过程中将一种或多种母体因素引入血腔中，以抑制宿主免疫。为了研究寄生后宿主免疫相关基因的早期变化，我们分析了寄生和未寄生、对照、寄主蛹的转录组。从非寄生和寄生宿主蛹中生成大约 17.7 和 1930 万个双端读段，并从头组装成 45,639 个转录本和 27,659 个非冗余单基因。 Unigene 的平均长度为 790 bp。共注释了 27,659 个 unigenes 中的 18,377 个，我们在寄生后 1 h 的宿主蛹中鉴定了 557 个差异表达的 unigenes，其中 21 个与免疫相关。寄生导致大多数模式识别受体下调和所有丝氨酸蛋白酶抑制剂上调。油菜疫霉的经切叶剖面在很大程度上受到寄生的影响。该研究为今后研究蛹虫草与其宿主菜青虫之间的分子相互作用提供了宝贵的资源。
The small cabbage butterfly, Pieris rapae, is an important pest of cruciferous corps, and Pteromalus puparum is a predominant pupal endoparasitoid wasp of this butterfly. For successful development of parasitoid offspring, female parasitoids usually introduce one or several kinds of maternal factors into the hemocoels during oviposition to suppress host immunity. To investigate the early changes in host immune-related genes following parasitization, we analyzed transcriptomes of parasitized and unparasitized, control, host pupae. Approximately 17.7 and 19.3 million paired-end reads were generated from nonparasitized and parasitized host pupae, and assembled de novo into 45,639 transcripts and 27,659 nonredundant unigenes. The average unigene length was 790 bp. A total 18,377 of 27,659 unigenes were annotated and we identified 557 differentially expressed unigenes in host pupae at 1 h after parasitization, of which 21 were immune-related. Parasitization led to downregulation of most pattern recognition receptors and upregulation of all serine protease inhibitors. The transcirptomic profile of P. rapae is considerably affected by parasitization. This study provides valuable sources for future investigations of the molecular interaction between P. puparum and its host P. rapae.