Graphene Oxide Based Recyclable in Vivo Device for Amperometric Monitoring of Interferon-γ in Inflammatory Mice
Graphene Oxide Based Recyclable in Vivo Device for Amperometric Monitoring of Interferon-γ in Inflammatory Mice
复制标题
基于氧化石墨烯的可回收体内装置用于电流监测炎症小鼠的干扰素-γ
DOI：
10.1021/acsami.8b13518
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发表时间：
2018-10-03
影响因子：
9.5
通讯作者：
Liu, Guozhen
中科院分区：
文献类型：
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作者：
Cao, Chaomin;Jin, Ronghua;Liu, Guozhen
细胞因子的检测具有挑战性，因为它们在生理条件下的丰度通常很低。纳米材料制备的界面在超灵敏传感方面显示出独特的优势。在这里，我们展示了一种基于氧化石墨烯(GO)和结构转换适配子的安培传感设备，用于长期检测活体中的细胞因子。该装置结合了单层GO，充当玻璃碳电极上的信号放大器。携带有氧化还原探针的针对干扰素-伽马的发夹适配子被共价连接，作为生物识别部分。干扰素-γ在释放捕获的氧化还原探针引入电化学信号的同时，能够触发适配子的构型变化。该体内检测装置能够定量、动态地检测细胞培养上清液中免疫细胞分泌的低至1.3pgmL(-1)的干扰素-γ，即使在高浓度的其他非特异性蛋白的情况下也不会出现基线漂移。这些生物兼容设备还被植入肠炎小鼠的皮下组织，在那里，他们在48小时内无需使用物理屏障或主动漂移校正算法就可以精确检测干扰素-γ。此外，即使在传感接口经过多轮再生后，该设备也可以重复使用。
Cytokine sensing is challenging due to their typically low abundances in physiological conditions. Nano material fabricated interfaces demonstrated unique advantages in ultrasensitive sensing. Here, we demonstrate an ampero-metric sensing device based on graphene oxide (GO) and structure-switching aptamers for long-term detection of cytokines in a living organism. The device incorporates a single layer of GO acting as a signal amplifier on glassy carbon electrodes. The hairpin aptamers specific to interferon-gamma (IFN-gamma), which were loaded with redox probes, are covalently attached to GO to serve as biorecognition moieties. IFN-gamma was able to trigger the configuration change of aptamers while releasing the trapped redox probes to introduce the electrochemical signal. This in vivo device was capable of quantitatively and dynamically detecting IFN-gamma down to 1.3 pg mL(-1) secreted by immune cells in cell culture medium with no baseline drift even at a high concentration of other nonspecific proteins. The biocompatible devices were also implanted into subcutaneous tissue of enteritis mice, where they performed precise detection of IFN-gamma over 48 h without using physical barriers or active drift correction algorithms. Moreover, the device could be reused even after multiple rounds of regeneration of the sensing interface.