Growth and denitrifying activity of Xanthobacter autotrophicus CECT 7064 in the presence of selected pesticides
Growth and denitrifying activity of Xanthobacter autotrophicus CECT 7064 in the presence of selected pesticides
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DOI：
10.1007/s00253-005-0182-8
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发表时间：
2006-07
影响因子：
5
通讯作者：
Florentina Sáez;C. Pozo;Miguel A. Gómez;M. V. Martínez-Toledo;B. Rodelas;J. González-López
中科院分区：
文献类型：
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作者：
Florentina Sáez;C. Pozo;Miguel A. Gómez;M. V. Martínez-Toledo;B. Rodelas;J. González-López
九种农业常用农药的应用效果（艾氏剂、林螨特、二甲双胍、甲基异草磷、甲基化、莠去津、西玛津、克菌丹和灭幼脲）对生长、CO2产生、黄曲霉自养菌CECT 7064菌株在培养基中的固氮活性[作为一氧化二氮（N2 O）释放]和亚硝酸盐积累（西班牙典型培养物保藏中心）（从浸没的固定膜中分离的微生物）进行研究。除草剂阿特拉津和杀虫剂二甲双胍完全抑制了该菌的生长和生物活性。在10 mg l-1的浓度下，其他测试的农药延迟了菌株CECT 7064的生长，但在培养96 h后并没有显著影响细菌的生长。X.除杀真菌剂克菌丹外，施用农药对自养型的影响都是负面的。几种农药（艾氏剂、林丹、甲草磷、甲氧苄脲和灭幼脲）对N_2O的释放有强烈的抑制作用，而杀虫双、莠去津和西玛津则完全抑制该菌株的固氮活性。探讨了农药对浸没式固定膜反应器净化效果的影响。
The effects of the application of nine pesticides used commonly in agriculture (aldrin, lindane, dimetoate, methylparathion, methidation, atrazine, simazine, captan and diflubenzuron) on growth, CO2production, denitrifying activity [as nitrous oxide (N2O) released] and nitrite accumulation in the culture medium byXanthobacter autotrophicusstrain CECT 7064 (Spanish Type Culture Collection) (a micro-organism isolated from a submerged fixed-film) were studied. The herbicide atrazine and the insecticide dimetoate totally inhibited growth and biological activity ofX. autotrophicusat 10 mg l−1, while the rest of the tested pesticides delayed the growth of strain CECT 7064 but did not drastically affect the bacterial growth after 96 h of culture. The denitrifying activity ofX. autotrophicuswas negatively affected by the pesticides application with the exception of fungicide captan. The release of N2O was strongly inhibited by several pesticides (aldrin, lindane, methylparathion, methidation and diflubenzuron), while dimetoate, atrazine and simazine inhibited totally the denitrifying activity of the strain. The effects of the pesticides on denitrifying submerged fixed-film reactor are discussed.