Electrospun collagen/chitosan nanofibrous membrane as wound dressing
Electrospun collagen/chitosan nanofibrous membrane as wound dressing
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DOI：
10.1016/j.colsurfa.2007.04.129
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发表时间：
2008-02-01
影响因子：
5.2
通讯作者：
Chen, Jan-Kan
中科院分区：
文献类型：
--
作者：
Chen, Jyh-Ping;Chang, Gwo-Yun;Chen, Jan-Kan
采用静电纺丝法制备了I型胶原、壳聚糖和聚环氧乙烷的复合纳米纤维膜，并通过戊二醛蒸汽进一步交联。通过扫描电子显微镜、扫描探针显微镜、共聚焦激光扫描显微镜、傅里叶变换红外光谱和拉伸试验对NFM进行详细分析。发现纳米纤维直径为134 +/-42nm，其在交联后增加到398 +/-76nm。交联后材料的杨氏模量有所增加，但拉伸强度、拉伸应变和吸水性能有所下降。NFM对3 T3成纤维细胞的生长无细胞毒性，具有良好的体外生物相容性。从动物研究来看，NFM在伤口愈合率方面优于纱布和市售胶原蛋白海绵伤口敷料。这种新型的静电纺丝基质将有潜力作为皮肤再生的伤口敷料。(c)2007 Elsevier B. V.保留所有权利。
Composite nanofibrous membranes (NFM) of type I collagen, chitosan, and polyethylene oxide was fabricated by electrospinning, which could be further crosslinked by glutaraldehyde vapor. The NFM was subject to detailed analysis by scanning electron microscopy, scanning probe microscopy, confocal laser scanning microscopy, Fourier transform infrared spectroscopy, and tensile testing. Nanofiber diameter was found to be 134 +/- 42 nm, which increased to 398 +/- 76 nm after crosslinking. The Young's modulus increased after crosslinking, however, the ultimate tensile strength, tensile strain, and water sorption capability decreased after crosslinking. NFMs showed no cytotoxicity toward growth of 3T3 fibroblasts and had good in vitro biocompatibility. From animal studies, the NFM was better than gauze and commercial collagen sponge wound dressing in wound healing rate. This novel electrospun matrix will have potential as a wound dressing for skin regeneration. (c) 2007 Elsevier B.V. All rights reserved.