A REAPPRAISAL OF THE USE OF DMSO FOR THE EXTRACTION AND DETERMINATION OF CHLOROPHYLLS-A AND CHLOROPHYLLS-B IN LICHENS AND HIGHER-PLANTS
A REAPPRAISAL OF THE USE OF DMSO FOR THE EXTRACTION AND DETERMINATION OF CHLOROPHYLLS-A AND CHLOROPHYLLS-B IN LICHENS AND HIGHER-PLANTS
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DOI：
10.1016/0098-8472(92)90034-y
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发表时间：
1992-04-01
影响因子：
5.7
通讯作者：
DAVISON, AW
中科院分区：
文献类型：
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作者：
BARNES, JD;BALAGUER, L;DAVISON, AW
本文重新评价了DMSO用于地衣和高等植物叶绿素a和B的提取和测定。由于纯叶绿素a和B在DMSO和80%丙酮中的吸收光谱之间的差异，重新确定了计算色素提取物中叶绿素a、叶绿素B和总（a + B）叶绿素的单独浓度的公式，以用于DMSO的特定用途。在地衣中，特别讨论了酸性地衣物质造成叶绿素降解的问题。用碳酸盐饱和的100%丙酮反复洗涤菌体，然后在含有PVP（i）的DMSO中提取，在从地衣中提取叶绿素的过程中使叶绿素向脱镁叶绿素的转化最小化，其中地衣物质的含量被表征。在地衣含有大量的酸性化合物，改进的DMSO测定证明优于80%丙酮的提取和测定叶绿素a和B浓度的上级。在一系列的高等植物，叶绿素a和B浓度的测定几乎是相同的，当修改的DMSO测定与传统的方法相比，叶绿素提取使用80%丙酮。此外，DMSO提取物可以冷藏长达7天，叶绿素a或B没有显著损失，或a/B比的变化。潜在的生态生理应用的改良DMSO测定，消除了研磨植物材料和植物提取物的必要性，进行了讨论。
The use of DMSO for the extraction and determination of chlorophylls a and b in lichens and higher plants was reevaluated. Because of differences between the absorption spectra of pure chlorophylls a and b in DMSO and 80% acetone, formulae to calculate the individual concentrations of chlorophyll a, chlorophyll b and total (a + b) chlorophyll in pigment extracts were redetermined for specific use with DMSO. In lichens, the problem of chlorophyll degradation resulting from the presence of acidic lichen substances was specifically addressed. Repeated washing of thalli with carbonate-saturated 100% acetone followed by extraction in DMSO containing PVP(i) minimized the conversion of chlorophylls to phaeophytin during extraction of chlorophylls from lichens for which the content of lichen substances was characterized. In lichens containing significant quantities of acidic compounds, the modified DMSO assay proved superior to 80% acetone for the extraction and determination of chlorophyll a and b concentrations. In a range of higher plants, determinations of chlorophyll a and b concentrations were virtually identical when the modified DMSO assay was compared with the traditional method of chlorophyll extraction using 80% acetone. Moreover, DMSO extracts could be cold-stored for up to 7 days with no significant loss of chlorophylls a or b, or changes in the a/b ratio. Potential eco-physiological applications of the modified DMSO assay, which eliminates the necessity for grinding plant material and centrifuging plant extracts, are discussed.