DI-3-N-Butylphthalide Alleviates the Blood-Brain Barrier Permeability of Focal Cerebral Ischemia Reperfusion in Mice
DI-3-N-Butylphthalide Alleviates the Blood-Brain Barrier Permeability of Focal Cerebral Ischemia Reperfusion in Mice
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DOI：
10.1016/j.neuroscience.2019.06.020
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发表时间：
2019-08-10
期刊：
影响因子：
3.3
通讯作者：
Gao, Xuan
中科院分区：
文献类型：
--
作者：
Li, Jiamin;Liu, Yang;Gao, Xuan
缺血性脑卒中发生在动脉闭塞和血流停止后，通过溶栓治疗恢复血液供应可能引起脑缺血再灌注损伤，导致血脑屏障（BBB）破坏。DI-3-n-butylphthalide （NBP）是从西芹籽中提取的提取物，对缺血性脑卒中具有神经保护作用。本研究探讨了NBP对脑IR引起的血脑屏障破坏以及紧密连接（TJs）相关蛋白和小泡转化的影响。我们的研究结果表明，NBP通过上调tj相关蛋白而下调caveolin-1来缓解脑ir诱导的血管通透性恶化。NBP显著改善IR后的神经功能和脑血流量，但减少脑水肿和梗死体积。综上所述，NBP通过降低脑梗死体积和神经功能缺损评分、降低脑水肿和血脑屏障通透性发挥神经保护作用。NBP的神经保护作用可能与其改善脑缺血区血流的能力有关。NBP可能成为预防缺血性脑卒中血脑屏障功能障碍的新型治疗药物。(c) 2019 ibroElsevier Ltd.出版。版权所有。
Ischemic stroke occurs following arterial occlusion and subsequent blood flow cease, and restoration of blood supply by thrombolytic therapy may cause cerebral ischemic reperfusion (IR) injury resulting in breakdowns of blood-brain barrier (BBB). DI-3-n-butylphthalide (NBP) is an extraction from Chinese celery Apium graveolens Linn seeds and has neuroprotective effects in ischemic stroke. This study explored effects of NBP on BBB disruption caused by cerebral IR and transformation of tight junctions (TJs)-associated proteins and caveolae. Our results demonstrated that NBP alleviated cerebral IR-induced deterioration of vascular permeability by up-regulating TJ-associated proteins but down-regulating caveolin-1. NBP significantly improved neurological function and cerebral blood flow but reduced cerebral edema and infarct volume after IR. In conclusion, NBP exerts neuroprotective effects through attenuating cerebral infarct volume and neurological deficit score, reducing cerebral edema and BBB permeability. The neuroprotective effect of NBP is possibly related to its ability to improve blood flow in cerebral ischemic areas. NBP may turn into a novel treatment drug to prevent BBB dysfunction in ischemic stroke. (C) 2019 IBRO. Published by Elsevier Ltd. All rights reserved.