Discovery of marine Bacillus species by 16S rRNA and rpoB comparisons and their usefulness for species identification
Discovery of marine Bacillus species by 16S rRNA and rpoB comparisons and their usefulness for species identification
复制标题
DOI：
10.1016/j.mimet.2009.01.003
复制
发表时间：
2009-04-01
影响因子：
2.2
通讯作者：
Qian, Pei-Yuan
中科院分区：
文献类型：
--
作者：
Ki, Jang-Seu;Zhang, Wen;Qian, Pei-Yuan
对芽孢杆菌群的系统研究偏向于陆地和致病分离株，而相对较少的研究检查了来自海洋环境的芽孢杆菌属物种。在这里，我们从不同的海洋环境中提取了 20 株芽孢杆菌菌株，并对它们的 165 rRNA 进行了测序。通过分子比较，我们将菌株分为 13 种芽孢杆菌基因型，并鉴定了 9 个物种：B. aquaemaris。 B. badius、B. cereus group、B. Firmus、B. halmapalus、B. hwajinpoensis、B. litoralis、B. sporothermodurans、B. vietmamensis 和三种难以区分的芽孢杆菌。此外，我们对 DNA 引导的 RNA 聚合酶 β 亚基 (rpoB) 基因进行了测序，并评估了其在识别芽孢杆菌方面的区分能力。芽孢杆菌 rpoB 基因的系统发育树根据其分类位置将每种芽孢杆菌分开，并得到统计支持。 rpoB 系统发育树上芽孢杆菌的分辨率大约是 16S rRNA 系统发育树上的 45 倍。这些结果表明，芽孢杆菌rpoB基因的多态性可用于芽孢杆菌属物种的鉴定，为芽孢杆菌属物种的鉴定提供了改进的方案。 (C) 2009 Elsevier B.V. 保留所有权利。
Systematic studies of the Bacillus group have been biased towards terrestrial and pathogenic isolates, and relatively few studies have examined Bacillus species from marine environments. Here we took twenty Bacillus strains from diverse marine environments and sequenced their 165 rRNA. Using molecular comparisons, we separated the strains into thirteen Bacillus genotypes and identified 9 species: B. aquaemaris. B. badius, B. cereus group, B. firmus, B. halmapalus, B. hwajinpoensis, B. litoralis, B. sporothermodurans, B. vietmamensis, and three indistinguishable Bacilli. In addition, we sequenced the DNA-directed RNA polymerase beta subunit (rpoB) gene and assessed its discriminative power in identifying Bacilli. Phylogenetic trees of Bacillus rpoB genes separated each Bacillus according to their taxonomic positions and were supported statistically. The resolution of Bacillus on the rpoB phylogenetic tree was approximately 45 times greater than on the 16S rRNA phylogenetic tree. These results demonstrate that the polymorphism of the Bacillus rpoB gene can be used to identify Bacillus species, providing an improved identification scheme for Bacillus species. (C) 2009 Elsevier B.V. All rights reserved.