Behavioral impairments and changes of nitric oxide and inducible nitric oxide synthase in the brains of molarless KM mice
Behavioral impairments and changes of nitric oxide and inducible nitric oxide synthase in the brains of molarless KM mice
复制标题
无臼齿KM小鼠大脑中一氧化氮和诱导型一氧化氮合酶的行为障碍和变化
DOI：
10.1016/j.bbr.2014.10.020
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发表时间：
2015-02-01
影响因子：
2.7
通讯作者：
Jiang, Qingsong
中科院分区：
文献类型：
--
作者：
Pang, Qian;Hu, Xingxue;Jiang, Qingsong
牙齿缺失是老年人的常见疾病，研究表明牙齿缺失会影响人类的认知功能，而一氧化氮（nitric oxide，NO）可能在牙齿缺失的认知功能中发挥重要作用。然而，根本的机制尚未确立。本研究的目的是评估KM小鼠磨牙缺失后的行为变化，以及在无磨牙条件下脑中NO和诱导型一氧化氮合酶（iNOS）的水平。据推测，测试的小鼠的磨牙缺失导致认知障碍，并且该过程通过脑中的NO通过信号通路介导。术后8周采用Morris水迷宫检测大鼠行为学变化。采用Griess法、免疫印迹法和免疫组化法检测NO和iNOS的变化。结果表明，磨牙缺失8周后，KM小鼠空间学习记忆能力下降，海马NO和iNOS水平升高。提示拔除磨牙与大鼠的行为障碍有关，海马NO和iNOS的变化可能参与了拔除磨牙后大鼠的行为改变。(C)2014爱思唯尔有限公司版权所有。
More studies showed that as a common disorder in senior population, loss of teeth could adversely affect human cognitive function, and nitric oxide (NO) might play an important role in the cognitive function. However, the underlying mechanism has not yet been well-established. The objectives of this study are to evaluate behavior changes of KM mice after loss of molars, and levels of NO and inducible nitric oxide synthase (iNOS) in the brain in molarless condition. It is hypothesized that loss of molars of the mice tested results in the cognitive impairments and that the process is mediated by NO in the brain through the signaling pathways. Morris water maze is used to test the behavioral changes after 8 weeks of the surgery. The changes of NO and iNOS are evaluated by using Griess assay, western blot, and immunohistochemistry method. The results show that 8 weeks after loss of molars, the spatial learning and memory of KM mice impair and the levels of NO and iNOS in mice hippocampus increase. These findings suggest that molar extraction is associated with the behavioral impairment, and that the changes of NO and iNOS in the hippocampus may be involved in the behavioral changes in the molarless condition. (C) 2014 Elsevier B.V. All rights reserved.