Survival by genotype: patterns at Mc1r are not black and white at the White Sands ecotone
Survival by genotype: patterns at Mc1r are not black and white at the White Sands ecotone
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DOI：
10.1111/mec.13894
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发表时间：
2017-01-01
影响因子：
4.9
通讯作者：
Rosenblum, E. B.
中科院分区：
文献类型：
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作者：
Des Roches, S.;Sollmann, R.;Rosenblum, E. B.
测量基因型、表型和野外生存之间的联系长期以来一直是适应性研究的焦点。我们进行了为期4年的捕获-再捕获研究，以衡量生存的基因型和表型在西南围栏Lingus（Sceloporus cowlesi）在白色沙滩交错带（过渡区之间的白色沙滩和黑暗的土壤栖息地）。我们报告了几个意想不到的发现。首先，与以前的工作表明，在S。来自沙丘中心的cowlesi与黑皮质素-1受体基因（Mc 1 r）的突变有关，cowlesi显示颜色表型和Mc 1 r基因型之间的关联最小。第二，Mc 1 r等位基因在过渡带S. Cowlesi似乎随着时间的推移而减少。第三，我们的捕获-再捕获数据显示，S。Cowlesi个体与衍生的Mc 1 r等位基因。因此，我们的研究结果表明，在过渡区的选择可能有利于野生型等位基因在最近几年。即使在一个系统中，基因型-表型关联似乎是黑色和白色，我们的研究表明，其他因素-包括表型可塑性，上位性，多效性和基因流-可能在白色沙滩交错区发挥重要作用。我们的研究强调了将分子、基因组和生物体方法联系起来以理解野生环境适应的重要性。此外，我们的研究结果表明，动态的自然选择，可以特别复杂的过渡栖息地，如生态交错带，并强调需要在未来的研究，探讨正在进行的选择在其他生态“灰色”地带的模式。
Measuring links among genotype, phenotype and survival in the wild has long been a focus of studies of adaptation. We conducted a 4-year capture-recapture study to measure survival by genotype and phenotype in the Southwestern Fence Lizard (Sceloporus cowlesi) at the White Sands ecotone (transition area between white sands and dark soil habitats). We report several unanticipated findings. First, in contrast with previous work showing that cryptic blanched coloration in S. cowlesi from the heart of the dunes is associated with mutations in the melanocortin-1 receptor gene (Mc1r), ecotonal S. cowlesi showed minimal association between colour phenotype and Mc1r genotype. Second, the frequency of the derived Mc1r allele in ecotonal S. cowlesi appeared to decrease over time. Third, our capture-recapture data revealed a lower survival rate for S. cowlesi individuals with the derived Mc1r allele. Thus, our results suggest that selection at the ecotone may have favoured the wild-type allele in recent years. Even in a system where a genotype-phenotype association appeared to be black and white, our study suggests that additional factors - including phenotypic plasticity, epistasis, pleiotropy and gene flow - may play important roles at the White Sands ecotone. Our study highlights the importance of linking molecular, genomic and organismal approaches for understanding adaptation in the wild. Furthermore, our findings indicate that dynamics of natural selection can be particularly complex in transitional habitats like ecotones and emphasize the need for future research that examines the patterns of ongoing selection in other ecological 'grey' zones.