Evaluation of Intestinal Microbial Metabolites in Preterm Infants with Different Initial Feeding Methods by In Vitro Fermentation Modeling System
Evaluation of Intestinal Microbial Metabolites in Preterm Infants with Different Initial Feeding Methods by In Vitro Fermentation Modeling System
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DOI：
10.3390/microorganisms10071453
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发表时间：
2022
期刊：
影响因子：
4.5
通讯作者：
Zhenghong Li
中科院分区：
文献类型：
--
作者：
Yunwei Li;Jingjing Jiang;Liying Zhu;Xin Wang;Weilin Wan;Danhua Wang;Zhenghong Li
Abstract: We aim to explore the intestinal microbial metabolites in preterm infants with noninvasive.methods and analyze the effects of initial feeding methods. Preterm infants with gestational weeks.lower than 34 were recruited for fecal sample collection every 7 days. Fecal pH, ammonia, bile acid,.and secretory IgA (sIgA) were tested. A 1:10 fecal slurry was inoculated into different culture media.containing different carbohydrates as the only carbon source: lactose (LAT), fructooligosaccharide.(FOS), galactooligosaccharide (GOS), and 20.-fucosyllactose (FL2). After 24 h of anaerobic culture.through an in vitro fermentation system, air pressure difference, carbohydrate degradation rate, and.short-chain fatty acids (SCFAs) content in fermentation pots were measured. Preterm infants were.assigned into two groups: group A, preterm infants fed by human milk, including mother’s own.milk and donor human milk (DHM); group B, preterm infants fed by preterm formula at first 3 days.and fed by human milk (including mother’s own milk and DHM) from day 4 to discharge. Group.A included 90 samples and group B included 70 samples. Group A had lower fecal pH (p = 0.023),.ammonia (p = 0.001), and bile acids (p = 0.025). Group B also had higher fecal sIgA levels, both in.OD (p = 0.046) and concentration (p < 0.0001) methods. Carbohydrates degradation rates in group A.were higher than group B, especially in LAT medium (p = 0.017) and GOS medium (p = 0.005). Gas.production amount had no significant difference in all four media. Several different SCFAs in four.kinds of different culture media in group A were higher than in group B, but valeric acid was lower.in group A. The initial feeding methods may affect the preterm infants’ intestinal microecology and.microbial metabolites for at least several weeks.
