Similarities in the General Chemical Composition of Colon Cancer Cells and Their Microvesicles Investigated by Spectroscopic Methods-Potential Clinical Relevance
Similarities in the General Chemical Composition of Colon Cancer Cells and Their Microvesicles Investigated by Spectroscopic Methods-Potential Clinical Relevance
复制标题
DOI：
10.3390/ijms21051826
复制
发表时间：
2020-03-01
影响因子：
5.6
通讯作者：
Baran, Jarek
中科院分区：
文献类型：
--
作者：
Depciuch, Joanna;Klebowski, Bartosz;Baran, Jarek
结肠癌占人类所有癌症病例的33%，大多数转移性结肠癌患者的预后仍然很差。癌症发展中的一个重要作用是癌症和正常细胞之间的沟通。这可能发生，除其他外，通过细胞外囊泡（包括微泡）（MV），这是由两种类型的细胞释放。MV可以调节多种生物过程，并被认为是有用的癌症生物标志物。在此，我们表明，结肠癌细胞和它们相应的肿瘤衍生微泡（TMV）之间的一般化学组成的相似性确实存在。这些结果已经通过光谱方法证实了四种结肠癌细胞系：HCT 116，LoVo，SW 480和SW 620，它们的侵袭性/转移潜力不同。我们的研究结果表明，拉曼和傅里叶变换拉曼光谱（FTIR）分析的细胞系和它们相应的TMV没有显着不同的表征其化学组成。然而，通过这两种方法获得的数据的分层聚类分析显示，只有拉曼光谱提供的结果符合结肠癌的分子分类，因此具有潜在的临床相关性。
Colon cancer constitutes 33% of all cancer cases in humans and the majority of patients with metastatic colon cancer still have poor prognosis. An important role in cancer development is the communication between cancer and normal cells. This may occur, among others, through extracellular vesicles (including microvesicles) (MVs), which are being released by both types of cells. MVs may regulate a diverse range of biological processes and are considered as useful cancer biomarkers. Herein, we show that similarity in the general chemical composition between colon cancer cells and their corresponding tumor-derived microvesicles (TMVs) does exist. These results have been confirmed by spectroscopic methods for four colon cancer cell lines: HCT116, LoVo, SW480, and SW620 differing in their aggressiveness/metastatic potential. Our results show that Raman and Fourier Transform InfraRed (FTIR) analysis of the cell lines and their corresponding TMVs did not differ significantly in the characterization of their chemical composition. However, hierarchical cluster analysis of the data obtained by both of the methods revealed that only Raman spectroscopy provides results that are in line with the molecular classification of colon cancer, thus having potential clinical relevance.