Effects of tributyltin (TBT) on enzyme activity and oxidative stress in hepatopancreas and hemolymph of small abalone, Haliotis diversicolor supertexta
Effects of tributyltin (TBT) on enzyme activity and oxidative stress in hepatopancreas and hemolymph of small abalone, Haliotis diversicolor supertexta
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三丁基锡（TBT）对小鲍鱼肝胰腺和血淋巴酶活性和氧化应激的影响
DOI：
10.1007/s00343-009-9222-z
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发表时间：
2009
影响因子：
--
通讯作者：
Yilei Wang
中科院分区：
文献类型：
--
作者：
Xiwei Jia;Ziping Zhang;Shuhong Wang;P. Lin;Z. Zou;Bangqin Huang;Yilei Wang
研究了三丁基锡（TBT）暴露对杂色鲍（Haliotis diversicolor supertexta）体内丙二醛（MDA）含量及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、酸性磷酸酶（ACP）和碱性磷酸酶（AKP）活性的影响。我们在暴露于0.35微克（锡）/升TBT后2、6、24、48、96和192小时采集肝胰腺和血淋巴样品。在肝胰腺中，接触三丁基锡化合物的动物在接触后2、24和96小时的ACP活性明显高于对照动物。AKP活性在2 h后也有所升高，但SOD和CAT活性没有变化。暴露后2小时和6小时，血淋巴中MDA浓度显着高于对照组。在接触三丁基锡化合物的动物血淋巴中，ACP活性在接触后192小时显著低于对照动物，而AKP活性在接触后2小时和192小时显著低于对照动物。血淋巴SOD活性和MDA水平在染毒后24 h显著低于对照组，但在染毒后96 h显著高于对照组。我们的研究结果表明，暴露于TBT引起ACP和AKP活性的快速变化，以及改变肝胰腺和血淋巴中MDA的浓度。超氧化物歧化酶（SOD）和过氧化氢酶（CAT）不参与小鲍肝胰腺对TBT的解毒过程。
We investigated the effect of tributyltin (TBT) exposure on the concentration of malondialdehyde (MDA) and the activity levels of the superoxide dismutase (SOD), catalase (CAT), and acid and alkaline phosphatase (ACP and AKP) enzymes in the small abalone, Haliotis diversicolor supertexta. We collected samples of the hepatopancreas and hemolymph 2, 6, 24, 48, 96, and 192 h after exposure to 0.35 µg (Sn)/L TBT. In the hepatopancreas, ACP activity was significantly higher in animals exposed to TBT 2, 24, and 96 h post-exposure compared with the control animals. AKP activity was also higher after 2 h, but SOD and CAT activity was unchanged. The concentration of MDA in the hemolymph was significantly higher than the control animals 2 and 6 h post-exposure. In the hemolymph of animals exposed to TBT, ACP activity was significantly lower than in the control animals 192 h post-exposure, whereas AKP activity was significantly lower 2 and 192 h post-exposure. Hemolymph SOD activity and levels of MDA were significantly lower than in the control animals 24 h after exposure but significantly higher after 96 h. Our results demonstrate that exposure to TBT cause rapid changes in ACP and AKP activity as well as altering the concentration of MDA in the hepatopancreas and hemolymph. SOD and CAT do not appear to be involved in the detoxification of TBT in the hepatopancreas of small abalone.