Reassortant H5N1 avian influenza viruses containing PA or NP gene from an H9N2 virus significantly increase the pathogenicity in mice
Reassortant H5N1 avian influenza viruses containing PA or NP gene from an H9N2 virus significantly increase the pathogenicity in mice
复制标题
含有H9N2病毒PA或NP基因的重配H5N1禽流感病毒显着增加对小鼠的致病性
DOI：
10.1016/j.vetmic.2016.07.002
复制
发表时间：
2016-08-30
影响因子：
3.3
通讯作者：
Liu, Xiufan
中科院分区：
文献类型：
--
作者：
Hao, Xiaoli;Hu, Jiao;Liu, Xiufan
不同流感病毒之间的重配是产生新型流感病毒的重要途径，其毒力和传播性不可预测，可能威胁公共健康。H9 N2和H5 N1亚型禽流感病毒在我国多个地区的家禽中流行，但它们之间存在自然重组的倾向。为了评估重组对公共卫生的风险，使用A/Goose/Jiangsu/k 0403/2010 [GS/10（H5 N1）]病毒作为骨架来产生一系列重组子，每个重组子包含来自A/Chicken/Jiangsu/WJ 57/2012 [WJ/57（H9 N2）]的优势S基因型的单个内部基因。接下来，我们评估了这些病毒的生物学特性，包括致病性、复制效率和聚合酶活性。我们发现亲本WI/57（H9 N2）和GS/10（H5 N1）病毒显示出高度的遗传相容性。值得注意的是，含有来自WJ/57（H9 N2）病毒的PA或NP基因的H5 N1抑制剂显著增加小鼠中的毒力和复制能力，以及显著增强聚合酶活性。我们的研究结果表明，H9 N2和H5 N1在家禽中的地方性极大地增加了通过重配产生新病毒的可能性，这可能对家禽业和公共卫生构成巨大威胁。(C)2016爱思唯尔B. V.保留所有权利。
Reassortment between different influenza viruses is a crucial way to generate novel influenza viruses with unpredictable virulence and transmissibility, which may threaten the public health. As currently in China, avian influenza viruses (AIVs) of H9N2 and H5N1 subtypes are endemic in poultry in many areas, while they are prone to reassort with each other naturally. In order to evaluate the risk of the reassortment to public health, A/Goose/Jiangsu/k0403/2010 [GS/10(H5N1)] virus was used as a backbone to generate a series of reassortants, each contained a single internal gene derived from the predominant S genotype of the A/Chicken/Jiangsu/WJ57/2012 [WJ/57(H9N2)]. We next assessed the biological characteristics of these assortments, including pathogenicity, replication efficiency and polymerase activity. We found that the parental WI/57(H9N2) and GS/10(H5N1) viruses displayed high genetic compatibility. Notably, the H5N1 reassortants containing the PA or NP gene from WJ/57(H9N2) virus significantly increased virulence and replication ability in mice, as well as markedly enhanced polymerase activity. Our results indicate that the endemicity of H9N2 and H5N1 in domestic poultry greatly increases the possibility of generating new viruses by reassortment that may pose a great threat to poultry industry and public health. (C) 2016 Elsevier B.V. All rights reserved.