Prokaryotic diversity in Zostera noltii-colonized marine sediments
Prokaryotic diversity in Zostera noltii-colonized marine sediments
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DOI：
10.1128/aem.66.4.1715-1719.2000
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发表时间：
2000-04-01
影响因子：
4.4
通讯作者：
Rodríguez-Valera, F
中科院分区：
文献类型：
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作者：
Cifuentes, A;Antón, J;Rodríguez-Valera, F
分析了浮游藻类栖息于底泥中的微生物多样性。提取微生物DNA，构建细菌和古生菌的16S rDNA克隆文库。这些样本的细菌多样性非常高，因为在分析的60个克隆中发现了57个不同的序列。文库中代表了领域细菌的八个主要谱系。最常被检索到的细菌群(36%的克隆)是与硫酸盐还原细菌相关的三角洲变形杆菌。第二丰富的类群(27%)是伽马变形杆菌，包括5个与S氧化内共生菌密切相关的克隆。古细菌克隆文库包括克伦考古塔和Eurya chaeota的成员，在分析的15个克隆中有9个不同的序列，表明与细菌有机体相比，多样性较低。这些序列没有一个与培养的古生物有密切的关系。
The diversity of microorganisms present in a sediment colonized by the phanerogam Zostera noltii has been analyzed. Microbial DNA was extracted and used for constructing two 16S rDNA clone libraries for Bacteria and Archaea. Bacterial diversity was very high in these samples, since 57 different sequences were found among the 60 clones analyzed. Eight major lineages of the Domain Bacteria were represented in the library. The most frequently retrieved bacterial group (36% Of the clones) was delta-Proteobacteria related to sulfate-reducing bacteria. The second most abundant group (27%) was gamma-Proteobacteria, including five clones closely related to S-oxidizing endosymbionts. The archaeal clone library included members of Crenarchaeota and Euryarchaeota, with nine different sequences among the 15 analyzed clones, indicating less diversity when compared to the Bacteria organisms. None of these sequences was closely related to cultured Archaea organisms.