Pan‐regional marine benthic cryptobiome biodiversity patterns revealed by metabarcoding Autonomous Reef Monitoring Structures
Pan‐regional marine benthic cryptobiome biodiversity patterns revealed by metabarcoding Autonomous Reef Monitoring Structures
复制标题
元条形码自主珊瑚礁监测结构揭示的泛区域海洋底栖隐生物多样性模式
作者：
J. Pearman;G. Chust;E. Aylagas;Ernesto Villarino;J. Watson;A. Chenuil;Á. Borja;A. Cahill;L. Carugati;R. Danovaro;R. David;X. Irigoien;I. Mendibil;S. Moncheva;N. Rodríguez‐Ezpeleta;M. Uyarra;S. Carvalho
自主珊瑚礁监测结构（ARMS）已在世界范围内应用于描述海洋底栖生物关键但经常被忽视的生物多样性模式。为了解开底栖生物模式中环境因素的相关性，在这里，通过标准化的元条形码协议，我们分析了固着和移动的（<2 mm）生物使用ARMS收集部署在六个区域不同的环境条件（3个站点× 3个重复每个区域）：波罗的海，西地中海，亚得里亚海，黑海和红海，比斯开湾。总共观察到27，473个扩增子序列变体（ASV），从黑海的1，404个到红海的9，958个。所有区域之间没有共享ASV。共享的ASV数量最多的是西地中海和亚得里亚海（116个）以及比斯开湾（115个）。在盐度较低的海洋（波罗的海和黑海）中，也有相对较多的反脊椎动物（103个），大多数与两栖类有关。我们发现，岩石海岸底栖生物的空间格局的组成差异，决定稍微比环境过滤的扩散限制。固着和移动的种群的扩散限制相似，固着种群的环境生态位宽度大于移动的种群。此外，我们的研究可以为未来评估隐生物群落的生物多样性模式提供基础，这可以贡献高达70%的当地生物多样性。
Autonomous Reef Monitoring Structures (ARMS) have been applied worldwide to characterize the critical yet frequently overlooked biodiversity patterns of marine benthic organisms. In order to disentangle the relevance of environmental factors in benthic patterns, here, through standardized metabarcoding protocols, we analyse sessile and mobile (<2 mm) organisms collected using ARMS deployed across six regions with different environmental conditions (3 sites × 3 replicates per region): Baltic, Western Mediterranean, Adriatic, Black and Red Seas, and the Bay of Biscay. A total of 27,473 Amplicon Sequence Variants (ASVs) were observed ranging from 1,404 in the Black Sea to 9,958 in the Red Sea. No ASVs were shared among all regions. The highest number of shared ASVs was between the Western Mediterranean and the Adriatic Sea (116) and Bay of Biscay (115). Relatively high numbers of ASVs (103), mostly associated with the genus Amphibalanus, were also shared between the lower salinity seas (Baltic and Black Seas). We found that compositional differences in spatial patterns of rocky‐shore benthos are determined slightly more by dispersal limitation than environmental filtering. Dispersal limitation was similar between sessile and mobile groups, while the sessile group had a larger environmental niche breadth than the mobile group. Further, our study can provide a foundation for future evaluations of biodiversity patterns in the cryptobiome, which can contribute up to 70% of the local biodiversity.