Humoral immune response to mycobacterial heat shock protein (hsp)65 in the cerebrospinal fluid of neuro‐Behçet patients
Humoral immune response to mycobacterial heat shock protein (hsp)65 in the cerebrospinal fluid of neuro‐Behçet patients
复制标题
Neuro-Behçet 患者脑脊液中分枝杆菌热休克蛋白 (hsp)65 的体液免疫反应
DOI：
10.1046/j.1365-2249.1998.00620.x
复制
发表时间：
1998
影响因子：
4.6
通讯作者：
G. Saruhan
中科院分区：
文献类型：
--
作者：
B. Taşcı;H. Direskeneli;P. Serdarog̃lu;G. Akman‐Demir;M. Eraksoy;G. Saruhan
尽管在Behçet‘s病(BD)中已经显示出对65-kD分枝杆菌hsp65(m-hsp65)的全身免疫反应，但局部免疫反应尚未被研究。对25例BD脑实质受累(p-NBD)、7例BD伴高颅压(ih-NBD)、8例无中枢神经系统(CNS)受累、30例多发性硬化(MS)和24例非炎症性中枢神经系统疾病(NIC)患者的血清和脑脊液(CSF)中抗m-hsp65抗体进行了检测。P-NBD患者脑脊液免疫球蛋白反应率(ELISA1.3 ± 0.9)显著高于NIC患者(0.7 ± 0.4，P <； 0.01)。P-NBD患者血清抗m-HSP65抗体阳性率为48%(12/25)，明显高于MS(3/30；P <； 0.03)和NIC(3/24；P <； 0.01)。脑脊液抗m-hsp65Ig G比值与BD病程相关(r = 为0.4，P<0.0 1； 为0.0 4)，与神经损害病程无关。Ih-NBD患者血清IgM和IgA反应升高，提示受累类型与p-NBD不同。这些结果提示p-NBD患者脑脊液对m-HSP65的局部体液反应增强，这可能与神经受累的发病机制有关。
Although systemic immune reactivity to 65‐kD mycobacterial hsp65 (m‐hsp65) has been shown previously in Behçet's disease (BD), local immune response was not investigated. We studied anti‐m‐hsp65 IgG, IgM and IgA antibodies in the serum and cerebrospinal fluid (CSF) of 25 BD patients with cerebral parenchymal involvement (p‐NBD), seven BD patients with intracranial hypertension (ih‐NBD), eight BD patients without central nervous system (CNS) involvement, 30 patients with multiple sclerosis (MS) and 24 patients with non‐inflammatory CNS disorders (NIC). Significantly higher CSF IgG responses were detected in p‐NBD patients (ELISA ratio 1.3 ± 0.9) compared with NIC (0.7 ± 0.4, P < 0.01). In p‐NBD patients' IgG, IgM or IgA CSF anti‐m‐hsp65 positivity rate was 48% (12/25); this was significantly higher when compared with MS (3/30; P < 0.03) and NIC (3/24; P < 0.01). CSF anti‐m‐hsp65 IgG ratios correlated with the duration of BD (r = 0.4, P < 0.04) but not with the duration of neurological involvement. Serum IgM and IgA responses were elevated in ih‐NBD, suggesting a different type of involvement than p‐NBD. These results implicate an increased local humoral response to m‐hsp65 in the CSF of p‐NBD patients, which might be related to the pathogenesis of neurological involvement.