ANTIBODIES TO MAJOR HISTOCOMPATIBILITY ANTIGENS PRODUCED BY HYBRID CELL LINES
ANTIBODIES TO MAJOR HISTOCOMPATIBILITY ANTIGENS PRODUCED BY HYBRID CELL LINES
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DOI：
10.1038/266550a0
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发表时间：
1977-01-01
期刊：
影响因子：
64.8
通讯作者：
HOWARD, JC
中科院分区：
文献类型：
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作者：
GALFRE, G;HOWE, SC;HOWARD, JC
骨髓瘤细胞和免疫供体脾细胞之间的融合已被证明是一种成功的方法，用于获得同质抗SRBC（抗绵羊红细胞）和抗TNP抗体1，2。这种方法的最强大的特征之一是，通过克隆，尽管使用非纯化的免疫原，也可以容易地获得合成单克隆抗体的细胞系。通过克隆技术解决了杂交细胞的异质群体的多个组分。这一特点使该系统成为研究复杂抗原结构的有力工具。已建立的细胞系提供了无限制的永久性材料供应的进一步优势，以及全球标准化的可能性。
FUSION between myeloma cells and spleen cells from immunised donors has been shown to be a successful method of deriving homogeneous anti-SRBC (anti-sheep red blood cell) and anti-TNP antibodies1,2. One of the most powerful features of this approach is that, by cloning, one may easily derive cell lines synthesising monoclonal antibodies despite using non-purified immunogens. The multiple components of a heterogeneous population of hybrid cells are resolved by cloning techniques. This feature makes the system a very powerful tool in the study of complex antigenic structures. The established cell lines offer the further advantage of unlimited permanent supply of material, and the possibility of worldwide standardisation.